Chlorogenic acid improves ex vivo vessel function and protects endothelial cells against HOCl-induced oxidative damage, via increased production of nitric oxide and induction of Hmox-1

• Published in: The Journal of nutritional biochemistry 2016-01, Vol.27, p.53-60
• Main Authors: Jiang, Rujia, Hodgson, Jonathan M., Mas, Emilie, Croft, Kevin D., Ward, Natalie C.
• Format: Article
• Language: English
• Subjects: Animals; Blood Vessels - drug effects; Blood Vessels - metabolism; Blood Vessels - physiopathology; Cells, Cultured; Chlorogenic acid; Chlorogenic Acid - metabolism; Chlorogenic Acid - pharmacology; Endothelial function; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Heme oxygenase-1; Humans; Hypochlorous Acid - toxicity; In Vitro Techniques; Mice; Mice, Inbred C57BL; Nitric oxide; Oxidative stress; Oxidative Stress - drug effects; Polyphenols
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2015.08.017

Dietary polyphenols are potential contributors toward improved cardiovascular health. Coffee is one of the richest sources of dietary polyphenols in a coffee-drinking population, the most abundant form being chlorogenic acid (CGA). Endothelial dysfunction is an early and major risk factor for cardiovascular disease. Nitric oxide (NO) is a key factor in regulation of endothelial function. Heme oxygenase-1 (Hmox-1), an inducible isoform of heme oxygenase that is produced in response to stressors such as oxidative stress, may also play a role in vascular protection. The aim of this study was to investigate the effect of CGA on endothelial function with oxidant-induced damage in isolated aortic rings from C57BL mice. We further examine the mechanism by investigating cell viability, activation of eNOS and induction of Hmox-1 in human aortic endothelial cells (HAECs). We found that pretreatment of isolated aortic rings with 10-μM CGA-protected vessels against HOCl-induced endothelial dysfunction (P