Understanding the role of clay minerals in the chromium(VI) bioremoval by Pseudomonas aeruginosa CCTCC AB93066 under growth condition: microscopic, spectroscopic and kinetic analysis

• Published in: World journal of microbiology & biotechnology 2015-11, Vol.31 (11), p.1765-1779
• Main Authors: Kang, Chunxi, Wu, Pingxiao, Li, Yuewu, Ruan, Bo, Li, Liping, Tran, Lytuong, Zhu, Nengwu, Dang, Zhi
• Format: Article
• Language: English
• Subjects: Adsorption; Aluminum Silicates - chemistry; Applied Microbiology; Bacteria; Batch Cell Culture Techniques; Biochemistry; Biodegradation, Environmental; Biomedical and Life Sciences; Bioremediation; Biota; Biotechnology; Carcinogens, Environmental - metabolism; Cell division; Cell morphology; Chromium; Chromium - metabolism; Clay; Clay minerals; Environmental conditions; Environmental Engineering/Biotechnology; Enzymes; Experiments; Fourier transforms; Glucose; Glucose - metabolism; Growth conditions; Higher education; Infrared spectroscopy; Kaolinite; Kinetics; Life Sciences; Metabolism; Microbiology; Microorganisms; Microscopy; Microscopy, Atomic Force; Minerals; Original Paper; Photoelectron Spectroscopy; Pseudomonas aeruginosa; Pseudomonas aeruginosa - growth & development; Pseudomonas aeruginosa - metabolism; Pseudomonas aeruginosa - ultrastructure; Reduction; Soil Pollutants - metabolism; Spectrum analysis; Vermiculite
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-015-1928-9

Laboratory batch experiments were conducted to investigate the role of clay minerals, e.g., kaolinite and vermiculite, in microbial Cr(VI) reduction by Pseudomonas aeruginosa under growth condition in glucose-amended mediums as a method for treating Cr(VI)-contaminated subsurface environment such as soil. Our results indicated that glucose could acted as an essential electron donor, and clay minerals significantly enhanced microbial Cr(VI) reduction rates by improving the consumption rate of glucose and stimulating the growth and propagation of P. aeruginosa . Cr(VI) bioreduction by both free cells and clay minerals-amended cells followed the pseudo-first-order kinetic model, with the latter one fitting better. The mass balance analyses and X-ray photoelectron spectroscopy analysis found that Cr(VI) was reduced to Cr(III) and the adsorption of total chromium on clay minerals-bacteria complex was small, implying that Cr(VI) bioremoval was not mainly due to the adsorption of Cr(VI) onto cells or clay minerals or clay minerals-cells complex but mainly due to the Cr(VI) reduction capacity of P. aeruginosa under the experimental conditions studied (e.g., pH 7). Atomic force microscopy revealed that the addition of clay minerals (e.g. vermiculite) decreased the surface roughness of Cr(VI)-laden cells and changed the cell morphology and dimension. Fourier transform infrared spectroscopy revealed that organic matters such as aliphatic species and/or proteins played an important role in the combination of cells and clay minerals. Scanning electron microscopy confirmed the attachment of cells on the surface of clay minerals, indicating that clay minerals could provide a microenvironment to protect cells from Cr(VI) toxicity and serve as growth-supporting materials. These findings manifested the underlying influence of clay minerals on microbial reduction of Cr(VI) and gave an understanding of the interaction between pollutants, the environment and the biota.