Full Deacylation of Polyethylenimine Dramatically Boosts Its Gene Delivery Efficiency and Specificity to Mouse Lung

High-molecular-mass polyethylenimines (PEIs) are widely used vectors for nucleic acid delivery. We found that removal of the residual N-acyl moieties from commercial linear 25-kDa PEI enhanced its plasmid DNA delivery efficiency 21 times in vitro, as well as 10,000 times in mice with a concomitant 1...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-04, Vol.102 (16), p.5679-5684
Main Authors: Thomas, Mini, Lu, James J., Ge, Qing, Zhang, Chengcheng, Chen, Jianzhu, Klibanov, Alexander M.
Format: Article
Language:English
Subjects:
Acylation
Animals
Biological Sciences
Cell Line, Tumor
Cultured cells
DNA
Gene expression
Gene Expression Regulation
Gene Transfer Techniques
Humans
Influenza, Human - drug therapy
Influenza, Human - genetics
Lung - metabolism
Lungs
Mice
Nitrogen
Orthomyxoviridae - drug effects
Orthomyxoviridae - metabolism
Plasmids
Polyethyleneimine - chemistry
Polyethyleneimine - metabolism
Polyethyleneimine - therapeutic use
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
RNA, Small Interfering - pharmacology
RNA, Small Interfering - therapeutic use
Small interfering RNA
Spleen
Transfection
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!