Isolation and characterization of microsatellite markers in Perilla frutescens Brit

We isolated and characterized 13 polymorphic microsatellite primers from Perilla frutescens Brit. var. frutescens by using a modified method that involves one‐way PCR amplification with single primer prior to enrichment with an ‘oligo hook’. The efficiency of this procedure for isolating unique micr...

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Bibliographic Details
Published in:Molecular ecology notes 2005-06, Vol.5 (2), p.455-457
Main Authors: Kwon, S.J, Lee, J.K, Kim, N.S, Yu, J.W, Dixit, A, Cho, E.G, Park, Y.J
Format: Article
Language:English
Subjects:
alleles
alleles per locus
DNA primers
enriched library
genetic markers
heterozygosity
loci
microsatellite repeats
microsatellites
molecular markers
molecular sequence data
nucleotide sequences
Perilla
Perilla frutescens
polymerase chain reaction
polymorphic loci
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Summary:We isolated and characterized 13 polymorphic microsatellite primers from Perilla frutescens Brit. var. frutescens by using a modified method that involves one‐way PCR amplification with single primer prior to enrichment with an ‘oligo hook’. The efficiency of this procedure for isolating unique microsatellite sequences was approximately 77%. The number of alleles per microsatellite locus ranged from three to 10 with an average of 6.5 alleles per locus while fragment size varied from 156 to 298 bp. The observed and expected heterozygosities ranged from 0.52 to 0.86 and 0.52 to 0.89, respectively. These newly isolated microsatellite markers are expected to provide valuable resources for different genetic studies currently underway in our Perilla genome research program.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00901.x