Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects

Increased production of reactive oxygen species (ROS) in vivo can lead to cellular biomolecule damage. Such damage has been suggested to contribute to the pathogenesis of insulin dependent diabetes mellitus (IDDM). In this study, we used the alkaline comet assay to measure DNA damage (single-strande...

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Bibliographic Details
Published in:Mutation research. DNA repair 2000-06, Vol.460 (1), p.53-60
Main Authors: Hannon-Fletcher, Mary P.A, O'Kane, Maurice J, Moles, Ken W, Weatherup, Colin, Barnett, Christopher R, Barnett, Yvonne A
Format: Article
Language:English
Subjects:
Alkaline comet assay
Biological and medical sciences
Chemical mutagenesis
DNA damage
Hba1c
IDDM
Medical sciences
Neutrophils
Toxicology
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Summary:Increased production of reactive oxygen species (ROS) in vivo can lead to cellular biomolecule damage. Such damage has been suggested to contribute to the pathogenesis of insulin dependent diabetes mellitus (IDDM). In this study, we used the alkaline comet assay to measure DNA damage (single-stranded DNA breaks and alkali-labile sites) in freshly isolated whole blood, lymphocytes, monocytes, and neutrophils from 23 subjects with IDDM and 32 age- and sex-matched controls. Analysis of the results showed elevated levels of DNA damage (expressed as % comet tail DNA) in the lymphocyte (4.10±0.47, 3.22±0.22), monocyte (4.28±0.47, 3.49±0.18), and whole blood (4.93±0.51, 4.51±0.23) fractions from IDDM subjects compared to controls, respectively, but the increases observed were not statistically significant. However, we found significantly elevated basal levels of DNA damage in the neutrophil fraction (8.38±0.64, 4.07±0.23; p
ISSN:0921-8777
1386-1476
DOI:10.1016/S0921-8777(00)00013-6