Localization of auxotrophic and benomyl resistance markers through the parasexual cycle in the Beauveria bassiana (Bals.) vuill entomopathogen

Genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the Beauveria bassiana deuteromycete were carried out using the parasexual cycle. Parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons...

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Bibliographic Details
Published in:Journal of invertebrate pathology 1998-09, Vol.72 (2), p.119-125
Main Authors: Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.), Paccola-Meirelles, L.D
Format: Article
Language:English
Subjects:
AUXOTROPHIC MARKERS
BEAUVERIA BASSIANA
BENOMILO
BENOMYL
Biological and medical sciences
CROSSBREDS
ESTABILIDAD GENETICA
FONGICIDE
Fundamental and applied biological sciences. Psychology
FUNGICIDAS
FUNGICIDE TOLERANCE
FUNGICIDES
Fungicides, Industrial
GENE LOCATION
GENETIC ANALYSIS
GENETIC MARKERS
GENETIC STABILITY
GENETICA
GENETICS
GENETIQUE
Genic rearrangement. Recombination. Transposable element
HETEROCARYON
LINKAGE GROUPS
LOCALISATION DE GENE
LOCALIZACION DE GENES
MARCADORES GENETICOS
MARQUEUR GENETIQUE
MITOSE
MITOSIS
Mitosporic Fungi - genetics
MITOTIC INSTABILITY
Molecular and cellular biology
Molecular genetics
PARASEXUALITY
PRODUCTOS DEL CRUZAMIENTO
PRODUIT DE CROISEMENT
RECOMBINACION
RECOMBINAISON
RECOMBINATION
Recombination, Genetic
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTANCE TO CHEMICALS
RESISTENCIA A PRODUCTOS QUIMICOS
SEGREGACION
SEGREGATION
SEX
SEXE
SEXO
STABILITE GENETIQUE
STRAINS
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Summary:Genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the Beauveria bassiana deuteromycete were carried out using the parasexual cycle. Parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons. Selection of the segregant products was made using two methodologies: total isolation and parental elimination. Colonies with recombinant traits were recovered directly from the heterocaryon in all crosses, and no diploid colony was isolated. This shows the high instability of the diploid nucleus in this species. Among the parasexual segregant products preferential recovery of markers in one of the parental strains involved in the crosses was detected, probably because of an inhibition of conidiogenesis from one parental strain to another. Genetic markers were localized in four linkage groups by the parasexual crosses. In the first group markers nic4, nic3, thi2, bio3, ade2, ths2, and ben1R were localized; in the second, the marker met1 in the third, pab1; and in the fourth, bio1. The parental strain 196/A11/3 is a carrier of translocation among the linkage groups I and III
ISSN:0022-2011
1096-0805
DOI:10.1006/jipa.1998.4768