survival mechanism of dune reed (Phragmites communis) cultures under high sodium chloride concentration

Adaptations to salt stress were studied in embryogenic cultures from two ecotypes of reed (Phragmites communisT.). In the 600 mM NaCl treatment, relative cell viability of dune reed embryogenic cultures from a desert region was 56% greater than the control, 198% greater than swamp reed embryogenic c...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2004-12, Vol.79 (3), p.291-298
Main Authors: Zhao, L.Q, Cui, S.X, Zhang, L.X, Zhang, C.L
Format: Article
Language:English
Subjects:
antioxidant activity
Biological and medical sciences
Biotechnology
catalase
cell growth
culture media
dose response
ecotypes
enzyme activity
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
genetic variation
in vitro culture
Methods. Procedures. Technologies
Miscellaneous
peroxidase
Phragmites australis
Phragmites communis
Plant cells and fungal cells
plasma membrane
salt stress
salt tolerance
sodium chloride
superoxide dismutase
viability
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Summary:Adaptations to salt stress were studied in embryogenic cultures from two ecotypes of reed (Phragmites communisT.). In the 600 mM NaCl treatment, relative cell viability of dune reed embryogenic cultures from a desert region was 56% greater than the control, 198% greater than swamp reed embryogenic cultures. After treatment with different NaCl concentrations, their relative growth rates (RGRs), pyridine nucleotides, activities of antioxidant enzymes and plasma membrane H+-ATPase (EC 3.6.1.35) were determined. The results showed that NADPH content, NADPH/NADP+ ratio and the activity of plasma membrane H+-ATPase in dune reed embryogenic cultures were higher than those of the control in the present of 600 mM NaCl. The activities of peroxidase (POD, EC 1.11.1.7) and catalase (CAT, EC 1.11.1.6) increased more in dune reed embryogenic cultures than in swamp reed embryogenic cultures. Dune reed embryogenic cultures tolerated higher concentration of NaCl than swamp reed embryogenic cultures. Under high concentration of NaCl, the survival of dune reed embryogenic cultures might be due to reductive status maintenance and ions absorption regulation in the plant cells. This phenomenon would be a result of cross-adaptation in nature.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-004-4610-8