Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei

Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factor...

Full description

Saved in:
Bibliographic Details
Published in:Cell biology international 2016-02, Vol.40 (2), p.214-222
Main Authors: Fu, Bo, Liu, Di, Ma, Hong, Guo, Zhen-Hua, Wang, Liang, Li, Zhong-Qiu, Peng, Fu-Gang, Bai, Jing
Format: Article
Language:English
Subjects:
Animals
Blastocyst - physiology
Cell Nucleus - metabolism
cloning efficiency
Cloning, Organism - methods
Embryo Transfer - methods
Embryonic Development - physiology
Embryos
Female
Fibroblasts - metabolism
nuclear factors
Nuclear Transfer Techniques - veterinary
Oocytes - metabolism
Oocytes - physiology
porcine oocytes
reprogram
somatic cell nuclear transfer
Swine - embryology
Swine - genetics
Swine - metabolism
tetraploid
Tetraploidy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramming. We introduced somatic cell nuclei into intact MII oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. We then examined the influence of the oocyte nucleus on tetraploid SCNT embryo development by assessing characteristics including pronucleus formation, cleavage rate, and blastocyst formation. Overall, tetraploid SCNT embryos have a higher developmental competence than do standard diploid SCNT embryos. Therefore, we have established an embryonic model in which a fetal fibroblast nucleus and an oocyte metaphase II plate coexist. Tetraploid SCNT represents a new research platform that is potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.10554