Enhanced survival of mice infused with bone marrow-derived as compared with adipose-derived mesenchymal stem cells

• Published in: Hepatology research 2015-12, Vol.45 (13), p.1353-1359
• Main Authors: Shiratsuki, Shogo, Terai, Shuji, Murata, Yasuhiko, Takami, Taro, Yamamoto, Naoki, Fujisawa, Koichi, Burganova, Guzel, Quintanilha, Luiz Fernando, Sakaida, Isao
• Format: Article
• Language: English
• Subjects: matrix metalloproteinase; mesenchymal stem cell; pro-coagulation; tissue factor
• ISSN: 1386-6346; 1872-034X
• DOI: 10.1111/hepr.12507

Aim Less invasive therapies using mesenchymal stem cells (MSC) are being developed to treat patients with severe liver cirrhosis. MSC constitute a promising cell source for regenerative therapy and are frequently isolated from bone marrow (BMSC) or adipose tissue (ASC). Therefore, this study assessed the characteristics of these two cell types and their safety for cell infusion. Methods In vitro, exhaustive genetic analysis was performed using human (h)BMSC and hASC. Subsequently, the expression of mRNA and protein was evaluated. In vivo, mouse (m)BMSC or mASC was infused into serial mice via the peripheral vein, and 24‐h survival rate, prothrombin time and cause of death were analyzed. Results On polymerase chain reaction, western blotting, enzyme‐linked immunoassay and fluorescence‐activated cell sorting, tissue factor was found to be expressed at higher levels in hASC than in hBMSC. Prothrombin time in mice infused with mASC (>120 s) was markedly longer than that of untreated mice (6.5 ± 1.7 s) and that of mice infused with BMSC (6.7 ± 0.8 s) (P