Effect of dry period dietary energy level in dairy cattle on volume, concentrations of immunoglobulin G, insulin, and fatty acid composition of colostrum

• Published in: Journal of dairy science 2016-02, Vol.99 (2), p.1515-1526
• Main Authors: Mann, S., Leal Yepes, F.A., Overton, T.R., Lock, A.L., Lamb, S.V., Wakshlag, J.J., Nydam, D.V.
• Format: Article
• Language: English
• Subjects: Animals; Cattle - physiology; colostrum; Colostrum - chemistry; dairy; Diet - veterinary; energy; Energy Intake; fatty acid; Fatty Acids - analysis; Female; Immunoglobulin G - analysis; insulin; Insulin - analysis; Lactation; Nutritional Requirements; Parity; Parturition; Pregnancy
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2015-9926

The objective was to investigate the effect of different dry cow feeding strategies on the volume, concentration of IgG and insulin, as well as fatty acid composition of colostrum. Our hypothesis was that different dry period diets formulated to resemble current feeding practices on commercial dairy farms and differing in plane of energy would have an effect on IgG and insulin concentration, as well as composition of fatty acid of colostrum. Animals (n=84) entering parity 2 or greater were dried off 57d before expected parturition and fed either a diet formulated to meet, but not greatly exceed energy requirements throughout the dry period (CON), or a higher energy density diet, supplying approximately 150% of energy requirements (HI). A third group received the same diet as group CON from dry-off until 29d before expected parturition. After this time point, from 28d before expected parturition until calving, they received a diet formulated to supply approximately 125% of energy requirements (I-med). Concentration of IgG and insulin in colostrum were measured by radial immunodiffusion and RIA, respectively. Composition of fatty acids was determined by gas-liquid chromatography. The IgG concentration was highest in colostrum of cows in group CON [96.1 (95% CI: 83.3–108.9) g/L] and lowest in group HI [72.4 (60.3–84.5) g/L], whereas insulin concentration was highest in group HI [1,105 (960–1,250) μU/mL] and lowest in group CON [853 (700–1,007) μU/mL]. Colostrum yield did not differ between treatments and was 5.9 (4.5–7.4), 7.0 (5.6–8.4), and 7.3 (5.9–8.7) kg in groups CON, I-med, and HI, respectively. A multivariable linear regression model showed the effect of dietary treatment group on IgG concentration was independent of the effect of dry matter. Cows in groups CON, I-med, and HI had an average colostral fat percentage of 5.0 (4.1–5.9), 5.6 (4.8–6.4), and 6.0 (5.2–6.8) and an average fat yield of 289 (196–380), 406 (318–495), and 384 (295–473) g, respectively. Colostrum of cows fed to exceed predicted energy requirements during the last 4wk of the dry period (groups I-med and HI) exhibited a higher concentration and yield of de novo fatty acid and lower concentrations but similar yield of preformed fatty acid compared with cows in group CON. Feeding a controlled energy diet during the dry period increased colostral IgG concentration in this study, whereas overall colostrum yield was not affected. Exceeding energy requirements during the period of colostrog