In Situ Supramolecular Assembly and Modular Modification of Hyaluronic Acid Hydrogels for 3D Cellular Engineering

A facile in situ supramolecular assembly and modular modification of biocompatible hydrogels were demonstrated using cucurbit[6]uril-conjugated hyaluronic acid (CB[6]-HA), diaminohexane-conjugated HA (DAH-HA), and tags-CB[6] for cellular engineering applications. The strong and selective host–guest...

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Bibliographic Details
Published in:ACS nano 2012-04, Vol.6 (4), p.2960-2968
Main Authors: Park, Kyeng Min, Yang, Jeong-A, Jung, Hyuntae, Yeom, Junseok, Park, Ji Sun, Park, Keun-Hong, Hoffman, Allan S, Hahn, Sei Kwang, Kim, Kimoon
Format: Article
Language:English
Subjects:
Animals
Assembly
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Biocompatible Materials - toxicity
Bridged-Ring Compounds - chemistry
Cell Engineering - methods
Cell Proliferation - drug effects
Cellular
Feasibility
Female
Fluorescein
Hyaluronic acid
Hyaluronic Acid - chemistry
Hyaluronic Acid - pharmacology
Hyaluronic Acid - toxicity
Hydrogels
Hydrogels - chemistry
Hydroxyapatite
Imidazoles - chemistry
Mice
Modular
NIH 3T3 Cells
Oligopeptides - chemistry
Polyamines - chemistry
Three dimensional
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Summary:A facile in situ supramolecular assembly and modular modification of biocompatible hydrogels were demonstrated using cucurbit[6]uril-conjugated hyaluronic acid (CB[6]-HA), diaminohexane-conjugated HA (DAH-HA), and tags-CB[6] for cellular engineering applications. The strong and selective host–guest interaction between CB[6] and DAH made possible the supramolecular assembly of CB[6]/DAH-HA hydrogels in the presence of cells. Then, the 3D environment of CB[6]/DAH-HA hydrogels was modularly modified by the simple treatment with various multifunctional tags-CB[6]. Furthermore, we could confirm in situ formation of CB[6]/DAH-HA hydrogels under the skin of nude mice by sequential subcutaneous injections of CB[6]-HA and DAH-HA solutions. The fluorescence of modularly modified fluorescein isothiocyanate (FITC)-CB[6] in the hydrogels was maintained for up to 11 days, reflecting the feasibility to deliver the proper cues for cellular proliferation and differentiation in the body. Taken together, CB[6]/DAH-HA hydrogels might be successfully exploited as a 3D artificial extracellular matrix for various tissue engineering applications.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn204123p