Simple Design for DNA Nanotubes from a Minimal Set of Unmodified Strands: Rapid, Room-Temperature Assembly and Readily Tunable Structure

DNA nanotubes have great potential as nanoscale scaffolds for the organization of materials and the templation of nanowires and as drug delivery vehicles. Current methods for making DNA nanotubes either rely on a tile-based step-growth polymerization mechanism or use a large number of component stra...

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Bibliographic Details
Published in:ACS nano 2013-04, Vol.7 (4), p.3022-3028
Main Authors: Hamblin, Graham D, Hariri, Amani A, Carneiro, Karina M. M, Lau, Kai L, Cosa, Gonzalo, Sleiman, Hanadi F
Format: Article
Language:English
Subjects:
Arrays
Backbone
Condensation polymerization
Crystallization - methods
Cyanine dyes
Deoxyribonucleic acid
DNA - chemistry
DNA - ultrastructure
Inclusions
Macromolecular Substances - chemistry
Materials Testing
Molecular Conformation
Nanocapsules - chemistry
Nanocapsules - ultrastructure
Nanostructure
Nanotubes
Nanotubes - chemistry
Nanotubes - ultrastructure
Particle Size
Porosity
Strands
Surface Properties
Temperature
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Summary:DNA nanotubes have great potential as nanoscale scaffolds for the organization of materials and the templation of nanowires and as drug delivery vehicles. Current methods for making DNA nanotubes either rely on a tile-based step-growth polymerization mechanism or use a large number of component strands and long annealing times. Step-growth polymerization gives little control over length, is sensitive to stoichiometry, and is slow to generate long products. Here, we present a design strategy for DNA nanotubes that uses an alternative, more controlled growth mechanism, while using just five unmodified component strands and a long enzymatically produced backbone. These tubes form rapidly at room temperature and have numerous, orthogonal sites available for the programmable incorporation of arrays of cargo along their length. As a proof-of-concept, cyanine dyes were organized into two distinct patterns by inclusion into these DNA nanotubes.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn4006329