Construction of an inducible transposon, INAc, to develop a gene tagging system in higher plants

An inducible transposable element, termed INAc (inducible Activator), was constructed for development of a gene tagging system in higher plants. The advantage of such an inducible element is that, unlike the native transposon, its excision can be induced at any time during plant development and the...

Full description

Saved in:
Bibliographic Details
Published in:Molecular breeding 2000-08, Vol.6 (4), p.353-367
Main Authors: Yuh-Chyang Charng, Pfitzner, Artur JP, Pfitzner, Ursula M, Charng-Chang, Kai-Fay, Chui-mei, Chen, Tu, Jenn, Tsong-Teh Kuo
Format: Article
Language:English
Subjects:
Flowers
Gene sequencing
gene tagging
Hygromycin
Lycopersicon esculentum
Marking
Molecular biology
Nicotiana tabacum
Plant biology
Plant species
Plants (botany)
Progeny
Salicylic acid
Tobacco
Tomatoes
Transposase
Transposase gene
Transposition
transposon INAc
Transposons
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An inducible transposable element, termed INAc (inducible Activator), was constructed for development of a gene tagging system in higher plants. The advantage of such an inducible element is that, unlike the native transposon, its excision can be induced at any time during plant development and the resulting mutants are stable after removal of the inducer. A fusion of the SA inducible promoter (PR-1a) with the Ac transposase gene was inserted together with a hygromycin resistance gene between ca. 400 bp sequences from each end of the maize Ac element, yielding INAc. The INAc element was introduced into tobacco and tomato plants. A high frequency of spontaneous transposition was apparent in primary transformed tomato calli but not in tobacco calli. Treatment of tobacco plants with salicylic acid induced transposition of INAc in both somatic and germinal tissue, with germinal transposition events being revealed by characterization of the progeny of transformed plants whose flowers were exposed to SA. The INAc element thus exhibits potential for development of an inducible transposon system suitable for gene isolation in heterologous plant species.
ISSN:1380-3743
1572-9788
DOI:10.1023/A:1009680105456