Mapping QTL associated with remobilization of zinc from vegetative tissues into grains of barley (Hordeum vulgare)

BACKGROUND AND AIMS: Limited remobilization of Zn from vegetative tissues into grains via phloem is a major physiological barrier against Zn loading into cereal grains. In present experiment, doubled-haploid mapping population (150 lines, derived from Clipper ×Sahara) of barley was genetically chara...

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Bibliographic Details
Published in:Plant and soil 2016-02, Vol.399 (1-2), p.193-208
Main Authors: Hussain, Shahid, Rengel, Zed, Mohammadi, Seyed A, Ebadi-Segherloo, Asghar, Maqsood, Muhammad A
Format: Article
Language:English
Subjects:
Agricultural soils
alleles
Barley
biofortification
Biomass
Biomedical and Life Sciences
breeding
chromosome mapping
Chromosomes
Developing countries
doubled haploids
Ecology
Flowering
Gene mapping
Genetic aspects
germplasm
Grain
Grains
Health aspects
Hordeum vulgare
LDCs
Leaves
Life Sciences
Nutritional aspects
Observations
Phenotypic traits
phloem
Plant biomass
Plant Physiology
Plant Sciences
Plant tissues
Quantitative trait loci
Regular Article
seeds
Soil Science & Conservation
Tissues
Zinc
Zinc in the body
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Summary:BACKGROUND AND AIMS: Limited remobilization of Zn from vegetative tissues into grains via phloem is a major physiological barrier against Zn loading into cereal grains. In present experiment, doubled-haploid mapping population (150 lines, derived from Clipper ×Sahara) of barley was genetically characterized for differential Zn remobilization. METHODS: The germplasm was grown under glasshouse conditions. Leaves (upper three), stem (the rest of the plant) and mature grains were sampled from the main-tillers at anthesis and maturity for Zn analysis. Quantitative trait loci (QTL) regulating time to anthesis, plant biomass, Zn concentration in vegetative tissues and remobilization of Zn from these tissues into grains were identified using a genetic linkage map of 485 markers. RESULTS: A significant variation existed in grain Zn concentration among the lines (27–75 μg Zn g⁻¹), and it correlated with the amount of Zn remobilized from vegetative tissues into grains. Sahara remobilized 37 % of pre-anthesis Zn reserves into grains; the presence of its alleles at all QTL associated with leaf (3 QTL) and stem (2 QTL) Zn remobilization increased the trait score. CONCLUSIONS: Present study provided an insight into the genetic basis of Zn remobilization from vegetative tissues into barley grains. Such information is useful in breeding for Zn biofortification.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-015-2684-1