Drug Testing in Blood: Validated Negative-Ion Chemical Ionization Gas Chromatographic-Mass Spectrometric Assay for Enantioselective Measurement of the Designer Drugs MDEA, MDMA, and MDA and Its Application to Samples from a Controlled Study with MDMA

• Published in: Clinical chemistry (Baltimore, Md.) Md.), 2005-10, Vol.51 (10), p.1811-1822
• Main Authors: Peters, Frank T, Samyn, Nele, Lamers, Caroline T.J, Riedel, Wim J, Kraemer, Thomas, de Boeck, Gert, Maurer, Hans H
• Format: Article
• Language: English
• Subjects: 3,4-Methylenedioxyamphetamine - analogs & derivatives; 3,4-Methylenedioxyamphetamine - blood; Chemicals; Chromatography; Dose-Response Relationship, Drug; Drug dosages; Drug-impaired driving; Ecstasy; Gas chromatography; Gas Chromatography-Mass Spectrometry - methods; Humans; Ingestion; Ionization; Mass spectrometry; N-Methyl-3,4-methylenedioxyamphetamine - blood; Plasma; Psychomotor performance; Sensitivity and Specificity; Stereoisomerism; Substance Abuse Detection - methods; Time Factors
• ISSN: 0009-9147; 1530-8561
• DOI: 10.1373/clinchem.2005.052746

The enantiomers of the designer drugs 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDEA) differ in their pharmacologic and toxicologic potency. The aim of this study was to develop an assay for measuring these enantiomers in small plasma volumes and to analyze samples from a controlled study with MDMA. The analytes were extracted from < or = 0.2 mL of plasma by mixed-mode solid-phase extraction. After derivatization with S-(-)-heptafluorobutyrylprolyl chloride, the resulting diastereomers were separated by gas chromatography (HP-5MS) within 17 min and detected by mass spectrometry in the negative-ion chemical ionization mode. The method was fully validated and applied to samples from a controlled study in which a single dose of racemic MDMA (75 mg) was administered. The derivatized enantiomers were well separated and detected with good sensitivity. The assay was linear (per enantiomer) at 1-50 microg/L for MDA and 5-250 microg/L for MDMA and MDEA. Analytical recovery, accuracy, repeatability, and intermediate precision data were within required limits. Extraction yields were 82.1%-95.3%. In the study samples, concentrations of R-(-)-MDMA significantly exceeded those of S-(+)-MDMA. Their ratios (R vs S) were always >1.0 and increased over time. Concentrations of S-(+)-MDA exceeded those of R-(-)-MDA, their ratios (R vs S) also increasing over time but remaining