Anti‐TCR Vβ‐specific DNA vaccination prolongs heart allograft survival in adult rats

Allospecific T cells are known to play a central role in the process of allograft rejection. Recently, it has been shown that T cell function could be specifically targeted using DNA vaccination. In our model, PCR analysis of the TCR‐β chain repertoire of T cells infiltrating rejected allografts sho...

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Bibliographic Details
Published in:European journal of immunology 2000-09, Vol.30 (9), p.2460-2464
Main Authors: Vignes, Caroline, Chiffoleau, Elise, Brouard, Sophie, Douillard, Patrice, Coudreuse, Damien, Soulillou, Jean‐Paul, Cuturi, Maria Cristina
Format: Article
Language:English
Subjects:
Allograft
DNA
Rejection
T cell
Vaccination
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Summary:Allospecific T cells are known to play a central role in the process of allograft rejection. Recently, it has been shown that T cell function could be specifically targeted using DNA vaccination. In our model, PCR analysis of the TCR‐β chain repertoire of T cells infiltrating rejected allografts showed specific expansions of the Vβ13 and Vβ2 families. In this study, we tested the effect on allograft survival of DNA vaccination against a specific TCR Vβ, in a model of heart allograft rejection in adult rats. Our results showed that anti‐TCR Vβ13 DNA vaccination lead to a significant prolongation of allograft survival compared to vaccination against other Vβ families or untreated recipients. The prolongation of allograft survival correlated in vitro with a decrease in anti‐donor reactivity of spleen cells from Vβ13‐vaccinated rats. These results show that, in a transplantation model, DNA vaccination could be used as a method to specifically manipulate a T cell response and thus prolong allograft survival.
ISSN:0014-2980
1521-4141
DOI:10.1002/1521-4141(200009)30:9<2460::AID-IMMU2460>3.0.CO;2-U