A method for isolating and culturing placental cells from failed early equine pregnancies

Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes....

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Bibliographic Details
Published in:Placenta (Eastbourne) 2016-02, Vol.38, p.107-111
Main Authors: Rose, B.V, Cabrera-Sharp, V, Firth, M.J, Barrelet, F.E, Bate, S, Cameron, I.J, Crabtree, J.R, Crowhurst, J, McGladdery, A.J, Neal, H, Pynn, J, Pynn, O.D, Smith, C, Wise, Z, Verheyen, K.L.P, Wathes, D.C, de Mestre, A.M
Format: Article
Language:English
Subjects:
Abortion, Veterinary - diagnostic imaging
Abortion, Veterinary - pathology
Animals
Cell culture
Cell Culture Techniques - methods
Cell Culture Techniques - veterinary
Cell Separation - methods
Cells, Cultured
Embryo Loss - diagnostic imaging
Embryo Loss - pathology
Embryo Loss - veterinary
Embryonic loss
Equine
Female
Gestational Age
Horses
Internal Medicine
Obstetrics and Gynecology
Placenta - diagnostic imaging
Placenta - pathology
Pregnancy
Pregnancy loss
Trophoblast
Trophoblasts - pathology
Ultrasonography, Prenatal - veterinary
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Summary:Abstract Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2015.12.014