Obesity‐driven alterations in adipose‐derived stem cells are partially restored by weight loss

Objective The therapeutic potential of adipose‐derived stem cells (ASCs) is reduced by various stress‐inducing conditions that affect tissue homeostasis such as diabetes, aging, and obesity. Previous works have provided evidence of negative effects of obesity on ASC populations, but it is unclear wh...

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Bibliographic Details
Published in:Obesity (Silver Spring, Md.) Md.), 2016-03, Vol.24 (3), p.661-669
Main Authors: Pérez, Laura M., Suárez, Javier, Bernal, Aurora, de Lucas, Beatriz, San Martin, Nuria, Gálvez, Beatriz G.
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - metabolism
Animals
Apoptosis
Cell Differentiation
Cell Proliferation
Flow cytometry
Homeostasis
Metabolism
Mice
Obesity
Obesity - metabolism
Polymerase chain reaction
Software
Stem cells
Stem Cells - metabolism
Studies
Subcutaneous Fat - metabolism
Weight control
Weight Loss
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Summary:Objective The therapeutic potential of adipose‐derived stem cells (ASCs) is reduced by various stress‐inducing conditions that affect tissue homeostasis such as diabetes, aging, and obesity. Previous works have provided evidence of negative effects of obesity on ASC populations, but it is unclear whether this persists after a weight loss. This study evaluated whether weight loss can restore the attenuated properties found in ASCs derived from populations with obesity (oASCs). Methods In vitro functional analyses were performed to investigate the possible recovery properties in mouse oASCs. Using ASCs isolated from subcutaneous tissue from formerly obese mice (dASCs) and control mice (cASCs), cell proliferation, viability, and some regenerative properties in these cells were analyzed compared with oASCs to evaluate the functional cell state. Results Cell proliferation, viability, and some regenerative properties are strengthened in dASCs and cASCs compared with oASCs. Nevertheless, metabolic analysis reveals a mitochondrial load misbalance and function leading to impaired respiration in dASCs. Conclusions This study demonstrates that an initial obese environment triggers a detrimental state in ASCs that is not completely recovered after weight loss.
ISSN:1930-7381
1930-739X
DOI:10.1002/oby.21405