Improved genome sequence of the phytopathogenic fungus Rhizoctonia solani AG1-IB 7/3/14 as established by deep mate-pair sequencing on the MiSeq (Illumina) system

•Resequencing of the R. solani AG1-IB 7/3/14 genome sequence on the Illumina MiSeq system improved its quality.•Improved gene prediction led to identification of new genes.•Comparative analyses revealed that each multicellular R. solani AG1-IB culture possesses individual characteristics. The phytop...

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Bibliographic Details
Published in:Journal of biotechnology 2015-06, Vol.203, p.19-21
Main Authors: Wibberg, Daniel, Rupp, Oliver, Jelonek, Lukas, Kröber, Magdalena, Verwaaijen, Bart, Blom, Jochen, Winkler, Anika, Goesmann, Alexander, Grosch, Rita, Pühler, Alfred, Schlüter, Andreas
Format: Article
Language:English
Subjects:
Assembly
AUGUSTUS
Base Sequence
Biotechnology
Brassica
DNA, Fungal
Fungi
Gene sequencing
Genome, Fungal
Genomes
High-Throughput Nucleotide Sequencing
Impact analysis
Plant pathogenic fungus
Rhizoctonia - genetics
Rhizoctonia solani
Rhizoctonia solani AG1-IB
Sequence Analysis, DNA
Soybeans
Strategy
Ultrafast sequencing
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Summary:•Resequencing of the R. solani AG1-IB 7/3/14 genome sequence on the Illumina MiSeq system improved its quality.•Improved gene prediction led to identification of new genes.•Comparative analyses revealed that each multicellular R. solani AG1-IB culture possesses individual characteristics. The phytopathogenic fungus Rhizoctonia solani AG1-IB of the phylum Basidiomycota affects various economically important crops comprising bean, rice, soybean, figs, cabbage and lettuce. The R. solani isolate 7/3/14 of the anastomosis group AG1-IB was deeply resequenced on the Illumina MiSeq system applying the mate-pair mode to improve its genome sequence. Assembly of obtained sequence reads significantly reduced the amount of scaffolds and improved the genome sequence of the isolate compared to the previous sequencing approach. The genome sequence of the AG1-IB isolate 7/3/14 now provides an up-graded basis to analyze genome features predicted to play a role in pathogenesis and for the development of strategies to antagonize the pathogenic impact of this fungus.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2015.03.005