Extraction and purification of a lectin from small black kidney bean (Phaseolus vulgaris) using a reversed micellar system

► Lectin from crude extract of small black kidney bean was extracted. ► The bioprocess was easy to perform using AOT in isooctane reversed micelles. ► The maximum protein recovery (53.28%) and purification factor (8.2-fold) were obtained. ► The efficiency of the process was confirmed by gel electrop...

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Bibliographic Details
Published in:Process biochemistry (1991) 2013-04, Vol.48 (4), p.746-752
Main Authors: He, Shudong, Shi, John, Walid, Elfalleh, Ma, Ying, Xue, Sophia Jun
Format: Article
Language:English
Subjects:
anionic surfactants
AOT/isooctane
Buffers
Extraction
gel electrophoresis
ionic strength
Kidney beans
Lectin
Lectins
Phaseolus vulgaris
potassium chloride
Protein recovery
Purification
Reverse micelles
sodium chloride
sodium phosphate
Strength
water content
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Summary:► Lectin from crude extract of small black kidney bean was extracted. ► The bioprocess was easy to perform using AOT in isooctane reversed micelles. ► The maximum protein recovery (53.28%) and purification factor (8.2-fold) were obtained. ► The efficiency of the process was confirmed by gel electrophoresis analysis. ► The lectin could be pre-purification by one step, with a low investment in equipment. Lectin from crude extract of small black kidney bean (Phaseolus vulgaris) was successfully extracted using the reversed micellar extraction (RME). The effects of water content of organic phase (Wo), ionic strength, pH, Aerosol-OT (AOT) concentration and extraction time on the forward extraction and the pH and ionic strength in the backward extraction were studied to optimize the extraction efficiency and purification factor. Forward extraction of lectin was found to be maximum after 15min of contact using 50mM AOT in organic phase with Wo 27 and 10mM citrate-phosphate buffer at pH 5.5 containing 100mM NaCl in the aqueous phase. Lectin was backward extracted into a fresh aqueous phase using sodium-phosphate buffer (10mM, pH 7.0) containing 500mM KCl. The overall yield of the process was 53.28% for protein recovery and 8.2-fold for purification factor. The efficiency of the process was confirmed by gel electrophoresis analysis.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2013.02.007