An investigation of the carbon nanotube – Lipid interface and its impact upon pulmonary surfactant lipid function

Abstract Multiwalled carbon nanotubes (MWCNTs) are now synthesized on a large scale, increasing the risk of occupational inhalation. However, little is known of the MWCNT-pulmonary surfactant (PS) interface and its effect on PS functionality. The Langmuir–Blodgett trough was used to evaluate the imp...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2015-07, Vol.55, p.24-32
Main Authors: Melbourne, Jodie, Clancy, Adam, Seiffert, Joanna, Skepper, Jeremy, Tetley, Teresa D, Shaffer, Milo S.P, Porter, Alexandra
Format: Article
Language:English
Subjects:
1,2-Dipalmitoylphosphatidylcholine - chemistry
Advanced Basic Science
Animals
Biomaterials
Compressing
Compressive Strength
Dentistry
Electron microscopy
Langmuir–Blodgett film
Lipid
Lipids
Lipids - chemistry
Lung
Lung - drug effects
Microscopy, Atomic Force
Microscopy, Electron
Microscopy, Electron, Transmission
Morphology
Nanoparticle
Nanoparticles - chemistry
Nanostructure
Nanotubes, Carbon - chemistry
Particle Size
Pulmonary Surfactants - chemistry
Rats
Rats, Sprague-Dawley
Risk
Surface Properties
Surface-Active Agents
Surfactants
Surgical implants
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Multiwalled carbon nanotubes (MWCNTs) are now synthesized on a large scale, increasing the risk of occupational inhalation. However, little is known of the MWCNT-pulmonary surfactant (PS) interface and its effect on PS functionality. The Langmuir–Blodgett trough was used to evaluate the impact of MWCNTs on fundamental properties of PS lipids which influence PS function, i.e. compression resistance and maximum obtainable pressure. Changes were found to be MWCNT length-dependent. ‘Short’ MWCNTs (1.1 μm, SD = 0.61) penetrated the lipid film, reducing the maximum interfacial film pressure by 10 mN/m (14%) in dipalmitoylphosphatidylcholine (DPPC) and PS, at an interfacial MWCNT-PS lipid mass ratio range of 50:1 to 1:1. ‘Long’ commercial MWCNTs (2.1 μm, SD = 1.2) caused compression resistance at the same mass loadings. ‘Very long’ MWCNTs (35 μm, SD = 19) sequestered DPPC and were squeezed out of the DPPC film. High resolution transmission electron microscopy revealed that all MWCNT morphologies formed DPPC coronas with ordered arrangements. These results provide insight into how nanoparticle aspect ratio affects the interaction mechanisms with PS, in its near-native state at the air–water interface.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2015.03.023