Distribution of Cryptosporidium species in Tibetan sheep and yaks in Qinghai, China

• Published in: Veterinary parasitology 2016-01, Vol.215, p.58-62
• Main Authors: Li, Pei, Cai, Jinzhong, Cai, Min, Wu, Wenxian, Li, Chunhua, Lei, Mengtong, Xu, Hailing, Feng, Lijun, Ma, Jiawen, Feng, Yaoyu, Xiao, Lihua
• Format: Article
• Language: English
• Subjects: Animals; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - parasitology; China - epidemiology; Cryptosporidiosis - epidemiology; Cryptosporidiosis - parasitology; Cryptosporidium; Cryptosporidium - classification; Cryptosporidium - genetics; Cryptosporidium andersoni; DNA, Protozoan - genetics; Feces - parasitology; Genotype; Qinghai; RNA, Protozoan - genetics; RNA, Ribosomal - genetics; Sheep; Sheep Diseases - epidemiology; Sheep Diseases - parasitology; SSU rRNA; Tibetan sheep; Yaks
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2015.11.009

•12.3% of 350 Tibetan sheep and 28.5% of 554 yaks examined were positive for Cryptosporidium spp.•C. xiaoi was the dominant species in Tibetan sheep.•Human-pathogenic C. ubiquitum XIIa subtype family was found in four Tibetan sheep.•C. andersoni, C. bovis, and C. ryanae cattle type were major species in yaks.•C. ryanae buffalo type and C. suis-like were found in yaks for the first time. Few data are available on the distribution of Cryptosporidium species in Tibetan sheep and yaks, which are free-range animals living in a cold, low oxygen, and high ultraviolet radiation habitat. In this study, 904 fecal specimens were collected from 350 Tibetan sheep and 554 yaks in six counties. Cryptosporidium spp. were detected and differentiated by PCR and sequence analyses. Altogether, 43 (12.3%) Tibetan sheep and 158 (28.5%) yaks were positive for Cryptosporidium spp. In Tibetan sheep, Cryptosporidium xiaoi (39/43, 90.7%) was the dominant species, with the remaining cases (4/43, 9.3%) by Cryptosporidium ubiquitum. All C. ubiquitum specimens belonged to the subtype family XIIa. In contrast, Cryptosporidium andersoni (72/158, 45.6%), Cryptosporidium bovis (47/158, 29.7%), Cryptosporidium ryanae cattle type (35/158, 22.2%), C. ryanae buffalo type (2/158, 1.3%), and Cryptosporidium suis-like (2/158, 1.3%) were identified in yaks. Contradictory to previous observations, C. andersoni was one of the dominant Cryptosporidium species in yaks in this study. Despite sharing habitats, Tibetan sheep and yaks are evidently infected with different Cryptosporidium species.