Biodiversity and in situ abundance of gut microflora of abalone ( Haliotis discus hannai) determined by culture-independent techniques

The compositions of bacterial communities in the gut of abalone were determined using the 16S rDNA clone library and fluorescence in situ hybridization (FISH). Sequencing of cloned 16S rDNA amplicons revealed a diverse community comprised Alpha-, Gamma- and Epsilonproteobacteria, and Mollicutes in t...

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Bibliographic Details
Published in:Aquaculture 2004-11, Vol.241 (1), p.453-463
Main Authors: Tanaka, Reiji, Ootsubo, Masashi, Sawabe, Tomoo, Ezura, Yoshio, Tajima, Kenichi
Format: Article
Language:English
Subjects:
16S rDNA clone library
Abalone
alpha-Proteobacteria
Animal aquaculture
Animal productions
Aquaculture
artificial diets
Bacteria
Bacterial community
Biodiversity
Biological and medical sciences
DNA libraries
epsilon-Proteobacteria
FISH
fluorescence in situ hybridization
Fundamental and applied biological sciences. Psychology
Fusobacteria
gamma-Proteobacteria
General aspects
Gut
Haliotis discus
Haliotis discus hannai
intestinal microorganisms
Laminaria
Marine
molecular sequence data
Mollicutes
mollusc culture
nucleotide sequences
phylogeny
Proteobacteria
ribosomal DNA
Vibrio
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Summary:The compositions of bacterial communities in the gut of abalone were determined using the 16S rDNA clone library and fluorescence in situ hybridization (FISH). Sequencing of cloned 16S rDNA amplicons revealed a diverse community comprised Alpha-, Gamma- and Epsilonproteobacteria, and Mollicutes in the gut of artificial diet-fed abalone, and majority of Mollicutes, Fusobacteria, Alpha- and Gammaproteobacteria in the gut of starved abalone. Biodiversity of gut bacterial community was rather high in artificial diet fed abalones than in Laminaria fed and starved animals. While, in situ abundance of the community composition determined by FISH revealed that 54% of 4′,6-diamidino-2-phenylindole (DAPI)-stained bacteria were hybridized with a probe for Gammaproteobacteria and 40% of DAPI-stained bacteria appeared to be of the Vibrio group. Alphaproteobacteria, which was frequent in clone libraries, was less abundant in artificial diet fed abalones determined by FISH. Our data show that the Vibrio group can be a dominant component in the gut microflora of abalone.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2004.08.032