Polyamines regulate cell growth and cellular methylglyoxal in high‐glucose medium independently of intracellular glutathione

Polyamines can presumably inhibit protein glycation, when associated with the methylglyoxal inevitably produced during glycolysis. Herein, we hypothesized a nonenzymatic interaction between putrescine and methylglyoxal in putrescine‐deficient or ‐overexpressing Dictyostelium cells in high‐glucose me...

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Bibliographic Details
Published in:FEBS letters 2016-03, Vol.590 (6), p.739-749
Main Authors: Kwak, Min‐Kyu, Lee, Mun‐Hyoung, Park, Seong‐Jun, Shin, Sang‐Min, Liu, Rui, Kang, Sa‐Ouk
Format: Article
Language:English
Subjects:
Culture Media
Dictyostelium - genetics
Dictyostelium - growth & development
Dictyostelium - metabolism
Dictyostelium discoideum
G1 Phase Cell Cycle Checkpoints
Genes, Protozoan
Glucose - metabolism
glutathione
Glutathione - metabolism
methylglyoxal
Ornithine Decarboxylase - genetics
Ornithine Decarboxylase - metabolism
polyamines
Polyamines - metabolism
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
putrescine
Putrescine - metabolism
Pyruvaldehyde - metabolism
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Summary:Polyamines can presumably inhibit protein glycation, when associated with the methylglyoxal inevitably produced during glycolysis. Herein, we hypothesized a nonenzymatic interaction between putrescine and methylglyoxal in putrescine‐deficient or ‐overexpressing Dictyostelium cells in high‐glucose medium, which can control methylglyoxal production. Putrescine was essentially required for growth rescue accompanying methylglyoxal detoxification when cells underwent growth defect and cell cycle G1‐arrest when supplemented with high glucose. Furthermore, methylglyoxal regulation by putrescine seemed to be a parallel pathway independent of the changes in cellular glutathione content in high‐glucose medium. Consequently, we suggest that Dictyostelium cells need polyamines for normal growth and cellular methylglyoxal regulation.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12102