Genetic & virulence profiling of ESBL-positive E. coli from nosocomial & veterinary sources

•blaCTX-M-14 upon plasmids of 25, 75, 100 & 150kb, and the chromosome of E. coli.•blaCTX-M-14 movement to new/re-organised genetic constructs during conjugation.•Clonal homology between clinical and veterinary E. coli specimens.•Veterinary E. coli found to be more virulent than clinical counterp...

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Bibliographic Details
Published in:Veterinary microbiology 2016-04, Vol.186, p.37-43
Main Authors: Tyrrell, J.M., Wootton, M., Toleman, M.A., Howe, R.A., Woodward, M., Walsh, T.R.
Format: Article
Language:English
Subjects:
Animals
Conjugation, Genetic
Cross Infection - microbiology
CTX-M-14
Drug Resistance, Bacterial - genetics
E. coli
ESBL (Extended spectrum beta-lactamases)
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli - pathogenicity
Escherichia coli Infections - microbiology
Humans
Pathogenicity
Plasmids - genetics
Virulence
Virulence - genetics
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Summary:•blaCTX-M-14 upon plasmids of 25, 75, 100 & 150kb, and the chromosome of E. coli.•blaCTX-M-14 movement to new/re-organised genetic constructs during conjugation.•Clonal homology between clinical and veterinary E. coli specimens.•Veterinary E. coli found to be more virulent than clinical counterparts.•Conjugative transfer of particular virulence genes. CTX-M genes are the most prevalent ESBL globally, infiltrating nosocomial, community and environmental settings. Wild and domesticated animals may act as effective vectors for the dissemination of CTX-producing Enterobacteriaceae. This study aimed to contextualise blaCTX-M-14-positive, cephalosporin-resistant Enterobacteriaceae human infections and compared resistance and pathogenicity markers with veterinary isolates. Epidemiologically related human (n=18) and veterinary (n=4) blaCTX-M-14-positive E. coli were fully characterised. All were typed by XbaI pulsed field gel electrophoresis and ST. Chromosomal/plasmidic locations of blaCTX-M-14 were deduced by S1-nuclease digestion, and association with ISEcp1 was investigated by sequencing. Conjugation experiments assessed transmissibility of plasmids carrying blaCTX-M-14. Presence of virulence determinants was screened by PCR assay and pathogenicity potential was determined by in vitro Galleria mellonella infection models. 84% of clinical E. coli originated from community patients. blaCTX-M-14 was found ubiquitously downstream of ISEcp1 upon conjugative plasmids (25–150kb). blaCTX-M-14 was also found upon the chromosome of eight E. coli isolates. CTX-M-14-producing E. coli were found at multiple hospital sites. Clonal commonality between patient, hospitals and livestock microbial populations was found. In vivo model survival rates from clinical isolates (30%) and veterinary isolates (0%) were significantly different (p
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2016.02.007