Polysaccharide from Angelica sinensis protects chondrocytes from H2O2-induced apoptosis through its antioxidant effects in vitro

This study aimed to explore the protective effects of Angelica sinensis polysaccharide (ASP) on rat chondrocyte injury induced by hydrogen peroxide (H2O2). Rat chondrocytes were cultured and treated with different concentrations of ASP alone or in combination with H2O2, and they were measured with c...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2016-06, Vol.87, p.322-328
Main Authors: Zhuang, Chao, Xu, Nan-Wei, Gao, Gong-Ming, Ni, Su, Miao, Kai-Song, Li, Chen-Kai, Wang, Li-Ming, Xie, Hong-Guang
Format: Article
Language:English
Subjects:
Angelica sinensis - chemistry
Angelica sinensis polysaccharide
Animals
Antioxidant
Antioxidants - isolation & purification
Antioxidants - pharmacology
Apoptosis - drug effects
Biomarkers - metabolism
Cell Survival - drug effects
Chondrocytes
Chondrocytes - cytology
Chondrocytes - drug effects
Chondrocytes - metabolism
Cytoprotection - drug effects
Down-Regulation - drug effects
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Male
Osteoarthritis
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Rat
Rats
Up-Regulation - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to explore the protective effects of Angelica sinensis polysaccharide (ASP) on rat chondrocyte injury induced by hydrogen peroxide (H2O2). Rat chondrocytes were cultured and treated with different concentrations of ASP alone or in combination with H2O2, and they were measured with cell viability, apoptosis, release of inflammatory cytokines, such as interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α), activity of superoxide dismutase (SOD), and catalase (CAT), and levels of malondialdehyde (MDA) production, respectively. In addition, quantitative real-time reverse transcription polymerase chain reaction was used to estimate the relative expression levels of osteoarthritis (OA)-associated genes, such as collagen type II (Col2a1), aggrecan, SOX9, matrix metalloproteinase (MMP)-1, -3, and -9, as well as tissue inhibitor of matrix metalloproteinase (TIMP)-1, respectively. Results indicated that ASP protected chondrocytes from H2O2-induced oxidative stress and subsequent cell injury through its antioxidant, antiapoptotic and anti-inflammatory effects in vitro. Our study suggests that ASP could become a therapeutic supplementation for the treatment of OA.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2016.02.031