Organ, Cellular, and Subcellular Localization of Brain-Specific Anion Transporter BSAT1

• Published in: Bulletin of experimental biology and medicine 2013-08, Vol.155 (4), p.491-497
• Main Authors: Baklaushev, V. P., Kardashova, K. Sh, Gurina, O. I., Yusubaliyeva, G. M., Zorkina, Ya. A., Chekhonin, V. P.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Animals, Outbred Strains; Antibodies, Monoclonal, Murine-Derived; Astrocytes - metabolism; Biomedical and Life Sciences; Biomedicine; Brain - cytology; Brain - metabolism; Cell Biology; Cell Line, Tumor; Female; Fluorescent Antibody Technique, Indirect; HEK293 Cells; Human Umbilical Vein Endothelial Cells - metabolism; Humans; Hybridomas; Internal Medicine; Laboratory Medicine; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Organ Specificity; Organic Cation Transport Proteins - chemistry; Organic Cation Transport Proteins - immunology; Organic Cation Transport Proteins - metabolism; Pathology; Peptide Fragments - immunology; Protein Transport; Rats
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-013-2186-1

Organ, cellular, and subcellular localization of brain-specific anion transporter BSAT1 was studied in rats using antibodies to the extracellular fragment (451-557 a.a). The antibodies were shown to recognize the antigen predominantly localized in the nervous tissue, tumors of glial origin, and primordial ovarian follicles. The absence of BSAT1 immunofluorescence signal in kidney and liver sections and accumulation of 125 I labeled antibodies to BSAT1 in these organs indicate that these antibodies do not cross-react with the most common isoforms of OATP expressed in these organs. Analysis of the cellular localization suggests that in the brain, BSAT1 is localized predominantly in astrocytes, but not in endothelial cells, as was previously reported. Laser scanning confocal microscopy with a set of relevant trackers revealed membrane localization of BSAT1. Taking into account the data on the of localization, we can conclude that antibodies to BSAT1 451-557 can be used for basic research of the transport of thyroxin and prostaglandins across the blood brain barrier and for testing the systems for targeted transport of diagnostic preparations and drugs across the blood brain barrier, e.g . to astroglial tumors.