Glucooligosaccharide production by Leuconostoc mesenteroides fermentation with efficient pH control, using a calcium hydroxide-sucrose solution

95.3% of the sucrose in a feed batch fermentation (300 g/L) was hydrolyzed by Leuconostoc mesenteroides subp. mesenteroides NRRL B-23188 glucansucrase. Further, the glucose of sucrose formed glucooligosaccharides (GOS) of degree of polymerization (DP) over 2, together with 91.6% of the maltose (200...

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Published in:Biotechnology and bioprocess engineering 2016, Vol.21 (1), p.39-45
Main Authors: Lee, Sun, Hanh, Nguyen Thi Thanh, Cho, Jae-Young, Kim, Ji Youn, Moon, Young Hwan, Yeom, Su-Cheong, Kim, Geun-Joong, Kim, Doman
Format: Article
Language:English
Subjects:
Bacteria
batch fermentation
Biotechnology
blood glucose
calcium
Calcium hydroxide
Carbohydrates
carbon
Carbon sources
Chemistry
Chemistry and Materials Science
Chromatography
E coli
Escherichia coli
Fermentation
glucooligosaccharides
Glucose
glucose tolerance tests
glycemic index
Industrial and Production Engineering
Leuconostoc mesenteroides
Lime
maltose
mice
Microorganisms
Natural products
polymerization
Process engineering
Research Paper
Salmonella
Streptococcus mutans
Studies
Sucrose
Yeast
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Summary:95.3% of the sucrose in a feed batch fermentation (300 g/L) was hydrolyzed by Leuconostoc mesenteroides subp. mesenteroides NRRL B-23188 glucansucrase. Further, the glucose of sucrose formed glucooligosaccharides (GOS) of degree of polymerization (DP) over 2, together with 91.6% of the maltose (200 g/L). Lime saccharate (lime sucrate) was used to control the pH during fermentation. The GOS products had DP between 2 and 7. When Streptococcus mutans mutansucrase (0.1 U/mL) reacted with 0.1% sucrose, addition of 0.1 ~ 10% GOS to the mutansucrase reaction digest resulted in a 56 ~ 90% reduction of mutan formation. GOS also reduced E. coli (72.2%) and Salmonella sp. (over 40.0%) growth, when 2.5% GOS was used as a single carbon source, compared to growth using glucose. The calculated glycemic index and glycemic load of GOS was 8 and 1, respectively, based on a 10 g carbohydrate serving. GOS was calculated to have 2.43 kcal/g. After a glucose tolerance test was performed using C57BL/6 mice, we found that mice treated with GOS showed a 59.4% lower increase in plasma glucose than those treated with maltose.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-015-0587-x