Physical and composition characteristics of clinical secretions compared with test soils used for validation of flexible endoscope cleaning

Summary Aim To determine which simulated-use test soils met the worst-case organic levels and viscosity of clinical secretions, and had the best adhesive characteristics. Methods Levels of protein, carbohydrate and haemoglobin, and vibrational viscosity of clinical endoscope secretions were compared...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of hospital infection 2016-05, Vol.93 (1), p.83-88
Main Authors: Alfa, M.J, Olson, N
Format: Article
Language:English
Subjects:
Adhesiveness
Bodily Secretions - chemistry
Carbohydrates - analysis
Cleaning
Clinical secretions
Decontamination - standards
Endoscopes
Equipment Contamination
Feces - chemistry
Gastrointestinal endoscopes
Humans
Infectious Disease
Proteins - analysis
Simulated use
Validation
Viscosity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Aim To determine which simulated-use test soils met the worst-case organic levels and viscosity of clinical secretions, and had the best adhesive characteristics. Methods Levels of protein, carbohydrate and haemoglobin, and vibrational viscosity of clinical endoscope secretions were compared with test soils including ATS, ATS2015, Edinburgh, Edinburgh-M (modified), Miles, 10% serum and coagulated whole blood. ASTM D3359 was used for adhesion testing. Cleaning of a single-channel flexible intubation endoscope was tested after simulated use. Results The worst-case levels of protein, carbohydrate and haemoglobin, and viscosity of clinical material were 219,828 μg/mL, 9296 μg/mL, 9562 μg/mL and 6 cP, respectively. Whole blood, ATS2015 and Edinburgh-M were pipettable with viscosities of 3.4 cP, 9.0 cP and 11.9 cP, respectively. ATS2015 and Edinburgh-M best matched the worst-case clinical parameters, but ATS had the best adhesion with 7% removal (36.7% for Edinburgh-M). Edinburgh-M and ATS2015 showed similar soiling and removal characteristics from the surface and lumen of a flexible intubation endoscope. Conclusions Of the test soils evaluated, ATS2015 and Edinburgh-M were found to be good choices for endoscope simulated-use test soils, as their composition and viscosity most closely matched worst-case clinical material.
ISSN:0195-6701
1532-2939
DOI:10.1016/j.jhin.2016.01.023