GUP1 and its close homologue GUP2, encoding multimembrane‐spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae

• Published in: Molecular microbiology 2000-07, Vol.37 (1), p.108-124
• Main Authors: Holst, Bjørn, Lunde, Christina, Lages, Fernanda, Oliveira, Rui, Lucas, Cândida, Kielland‐Brandt, Morten C.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Biological Transport, Active; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Culture Media; DNA Transposable Elements; Ethanol - metabolism; Gene Deletion; Genes, Fungal; glycerol; Glycerol - metabolism; GUP1 gene; GUP1 protein; GUP2 gene; GUP2 protein; Kinetics; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane Proteins - metabolism; Membrane Transport Proteins; Molecular Sequence Data; Mutagenesis, Insertional; Phenotype; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins; Sequence Alignment; Subcellular Fractions
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1046/j.1365-2958.2000.01968.x

Many yeast species can utilize glycerol, both as a sole carbon source and as an osmolyte. In Saccharomyces cerevisiae, physiological studies have previously shown the presence of an active uptake system driven by electrogenic proton symport. We have used transposon mutagenesis to isolate mutants affected in the transport of glycerol into the cell. Here we present the identification of YGL084c, encoding a multimembrane‐spanning protein, as being essential for proton symport of glycerol into S. cerevisiae. The gene is named GUP1 (glycerol uptake) and, for growth on glycerol, is important as a carbon and energy source. In addition, in strains deficient in glycerol production it also provides osmotic protection by the addition of glycerol. Another open reading frame (ORF), YPL189w, presenting a high degree of homology to YGL084c, similarly appears to be involved in active glycerol uptake in salt‐containing glucose‐based media in strains deficient in glycerol production. Analogously, this gene is named GUP2. To our knowledge, this is the first report on a gene product involved in active transport of glycerol in yeasts. Mutations with the same phenotypes occurred in two other ORFs of previously unknown function, YDL074c and YPL180w.