The use of Biochip immunofluorescence microscopy for the serological diagnosis of epidermolysis bullosa acquisita

Epidermolysis bullosa acquisita is a rare autoimmune bullous disease characterized by the presence of circulating antibodies directed against the collagen type VII. Diagnosis is generally based on clinical history, clinical features, histology, direct and indirect immunofluorescence, immunoblotting...

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Bibliographic Details
Published in:Archives of Dermatological Research 2016-05, Vol.308 (4), p.273-276
Main Authors: Marzano, Angelo Valerio, Cozzani, Emanuele, Biasin, Matteo, Russo, Irene, Alaibac, Mauro
Format: Article
Language:English
Subjects:
Autoantibodies - blood
Autoantibodies - immunology
Cell Line
Collagen Type VII - immunology
Concise Communication
Dermatology
Enzyme-Linked Immunosorbent Assay
Epidermolysis Bullosa Acquisita - diagnosis
Female
HEK293 Cells
Humans
Male
Medicine
Medicine & Public Health
Microscopy, Fluorescence
Pilot Projects
Protein Array Analysis - methods
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Summary:Epidermolysis bullosa acquisita is a rare autoimmune bullous disease characterized by the presence of circulating antibodies directed against the collagen type VII. Diagnosis is generally based on clinical history, clinical features, histology, direct and indirect immunofluorescence, immunoblotting and ELISA. Our study aims to determine the validity of the Biochip immunofluorescence microscopy for the serological diagnosis of epidermolysis bullosa acquisita. Six patients with epidermolysis bullosa acquisita and presence of antibodies against type VII collagen confirmed by ELISA were included in the study. Subsequently, all sera of patients were analyzed using Biochip. Antibodies anti-collagen type VII were detected in all sera by means of the Biochip technology. Thus, Biochip shows a good correlation with ELISA and seems to be an appropriate method for the diagnosis of epidermolysis bullosa acquisita. It is an easy, fast and standardized method which could facilitate the diagnosis of this autoimmune bullous disease. We suggest that it could be used as an initial screening test to identify patients with epidermolysis bullosa acquisita.
ISSN:0340-3696
1432-069X
DOI:10.1007/s00403-016-1632-0