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Synergism of ABC transporter modulators and agricultural fungicides in control of Botrytis cinerea

ABC transporters (=ATP binding cassette transporters) are membrane-bound proteins that utilise ATP to transport a wide spectrum of compounds through biological membranes. Simultaneous or multi drug resistance (MDR) to several classes of agricultural fungicides depends on their decreased accumulation...

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Bibliographic Details
Published in:Journal of plant pathology 2004-12, Vol.86 (4), p.301-301
Main Authors: Carrideo, G, Lanzotti, V, Barile, E, Del Sorbo, G
Format: Article
Language:English
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Summary:ABC transporters (=ATP binding cassette transporters) are membrane-bound proteins that utilise ATP to transport a wide spectrum of compounds through biological membranes. Simultaneous or multi drug resistance (MDR) to several classes of agricultural fungicides depends on their decreased accumulation at the level of target site, as a consequence of energy-dependent efflux operated by ABC transporters. To reduce application rates of agricultural fungicides, we tested the effectiveness of two classes of non-toxic compounds, which inhibit ABC transporters: jatrophane diterpenes from Euphorbia sp. and the plant metabolite prenylchrysin. These compounds show strong inhibitory activity on the main human ABC transporter, P-glycoprotein, and the multidrug transporter Pdr5p of Saccharomyces cerevisiae, and show ability to reverse the MDR phenotype. These ABC transporter modulators were used in combination to test their synergism with a number of agricultural fungicides (tebuconazole, imazalil, fludioxonil, fenpiclonil, tolylfluanid and fenhexamid) in germ tube elongation inhibition tests on Botrytis cinerea. Results of in vitro experiments showed a clear synergism of jatrophanes with fludioxonil and tebuconazole and of prenyl-chrysin with tolylfluanid, whereas an antagonistic effect was observed between prenyl-chrysin and fludioxonil. Tests on tomato and bean leaves artificially inoculated with B. cinerea conidia confirmed the synergism of jatrophanes with tebuconazole and tolylfluanid with prenyl-chrysin.
ISSN:1125-4653