Simultaneous Determination of Sulfonamides, Penicillins and Coccidiostats in Pork by High-Performance Liquid Chromatography-Tandem Mass Spectrometry

• Published in: Journal of chromatographic science 2012-05, Vol.50 (5), p.414-425
• Main Authors: Nebot, C., Regal, P., Miranda, J., Cepeda, A., Fente, C.
• Format: Article
• Language: English
• Subjects: Animals; Antiprotozoal Agents - analysis; Biological and medical sciences; Chromatography; Chromatography, High Pressure Liquid - economics; Chromatography, High Pressure Liquid - methods; Coccidiostats - analysis; Drugs; Food industries; Fundamental and applied biological sciences. Psychology; Liquids; Mass spectrometry; Mathematical analysis; Meat - analysis; Meat and meat product industries; Penicillins - analysis; Pork; Residues; Sensitivity and Specificity; Sulfonamides - analysis; Swine; Tandem Mass Spectrometry - economics; Tandem Mass Spectrometry - methods; Veterinary; Veterinary Drugs - analysis
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/bms021

Veterinary drugs are widely and legally used to treat and prevent disease in livestock. However, drugs are also used illegally as growth-promoting agents. To protect the health of consumers, maximum residue limits (MRL) in food of animal origin have been established and are listed in Regulation 37/2010. According to this regulation, more than 300 drugs need to be controlled regularly in laboratories for residues of veterinary drugs. A cost-effective analytical method is very important and explains why the development of multi-residual methods is becoming popular in laboratories. The aim of this work is to describe a simple, rapid and economical high-performance liquid chromatography-tandem mass spectrometry method for the simultaneous identification and quantification of 21 veterinary drugs in pork muscle samples. The sample clean-up procedure is performed with acidified dichloromethane and does not require solid phase extraction. The method is applicable to nine sulfonamides and seven coccidiostats identified within 36 min. Calculated relevant validation parameters such as recoveries (from 72.to 126 %), intra-precision and intermediate precision (relative standard deviation below 40 %) and decision limits (below 7 µg Kg−1) were within acceptable range and in compliance with the requirements of Commission Decision 2002/657/EC.