Hydrogen peroxide biosensor utilizing a hybrid nano-interface of iron oxide nanoparticles and carbon nanotubes to assess the quality of milk

• Published in: Sensors and actuators. B, Chemical Chemical, 2015-08, Vol.215, p.166-173
• Main Authors: Thandavan, Kavitha, Gandhi, Sakthivel, Nesakumar, Noel, Sethuraman, Swaminathan, Rayappan, John Bosco Balaguru, Krishnan, Uma Maheswari
• Format: Article
• Language: English
• Subjects: Biosensors; Carbon nanotubes; Catalase; Electrical measurements; Electrochemical bio-sensor; Hybrid interface; Hydrogen peroxide; Iron oxide; Iron oxides; Milk; Milk analysis; Nanostructure; Sensors
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2015.03.041

The nano hybrid interface accelerates the direct electron transfer from the catalase to the electrode in a mediator-free environment and thus accentuates for the accurate sensing of hydrogen peroxide along with a very quick response time of less than 1s. •Design of catalase based amperometric biosensor with a hybrid nanointerface of iron oxide and carbon nanotubes.•Linear range of hydrogen peroxide was obtained from 1.2 to 21.6μM.•A quick response time of less than 1s.•Reproducible, stable and interferent free system was obtained.•The LOD and LOQ were found to be 3.7nM and 12.2nM respectively.•Successfully used for the detection of hydrogen peroxide in the presence of milk samples. A hybrid interface was developed using nano iron oxide and carbon nanotubes and this architecture offered an improved performance for the detection of hydrogen peroxide. Nano iron oxide was synthesized by a simple thermal co-precipitation technique and it was dispersed in nafionic solution. To this mixture added the catalase enzyme adsorbed multi-walled carbon nanotubes and this solution was used for the modification of the electrode. The morphology of the prepared nanocomposite was observed using FE-TEM and the electrochemical studies were carried out using cyclic voltammetry and amperometry. The linear range of the prepared amperometric sensor was found to be between 1.2 and 21.6μM with a quick response time of less than 1s. The interference, reproducibility and stability studies were carried out with satisfactory results. The limit of detection and limit of quantification were found to be 3.7nM and 12.2nM respectively. With the convincing results obtained in terms of the performance of the biosensor, this platform was successfully upgraded for the determination of hydrogen peroxide in the presence of milk samples.