Light-Triggered Capture and Release of DNA and Proteins by Host-Guest Binding and Electrostatic Interaction

The development of an effective and general delivery method that can be applied to a large variety of structurally diverse biomolecules remains a bottleneck in modern drug therapy. Herein, we present a supramolecular system for the dynamic trapping and light‐stimulated release of both DNA and protei...

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Bibliographic Details
Published in:Chemistry : a European journal 2015-02, Vol.21 (8), p.3271-3277
Main Authors: Moratz, Johanna, Samanta, Avik, Voskuhl, Jens, Mohan Nalluri, Siva Krishna, Ravoo, Bart Jan
Format: Article
Language:English
Subjects:
Azo Compounds - chemistry
Biomolecules
Charging
Chemistry
Chemotherapy
Cyclodextrin
Cyclodextrins
Cyclodextrins - chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - metabolism
Dynamical systems
Electrostatic properties
Electrostatics
host-guest systems
Light
molecular recognition
Molecular Structure
Protein Binding
Proteins
Proteins - chemistry
Proteins - metabolism
self-assembly
Static Electricity
Vesicles
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Summary:The development of an effective and general delivery method that can be applied to a large variety of structurally diverse biomolecules remains a bottleneck in modern drug therapy. Herein, we present a supramolecular system for the dynamic trapping and light‐stimulated release of both DNA and proteins. Self‐assembled ternary complexes act as nanoscale carriers, comprising vesicles of amphiphilic cyclodextrin, the target biomolecules and linker molecules with an azobenzene unit and a charged functionality. The non‐covalent linker binds to the cyclodextrin by host–guest complexation with the azobenzene. Proteins or DNA are then bound to the functionalized vesicles through multivalent electrostatic attraction. The photoresponse of the host–guest complex allows a light‐induced switch from the multivalent state that can bind the biomolecules to the low‐affinity state of the free linker, thereby providing external control over the cargo release. The major advantage of this delivery approach is the wide variety of targets that can be addressed by multivalent electrostatic interaction, which we demonstrate on four types of DNA and six different proteins. Cyclodextrin vesicles bind charged biomolecules, such as DNA or proteins, in the presence of light‐responsive charged guest molecules. The complexes can be disassembled under UV‐light irradiation based on trans/cis isomerization of the azobenzene guests (see figure).
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.201405936