UPLC–MS/MS Method for Determination of Bepotastine in Human Plasma

A sensitive and rapid method for quantitation of bepotastine in human plasma has been established using ultra performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC–ESI–MS/MS). Valsartan was used as an internal standard. Bepotastine and internal standard in plasma s...

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Bibliographic Details
Published in:Journal of chromatographic science 2014-09, Vol.52 (8), p.886-893
Main Authors: Choi, Yun Kyoung, Chung, Yoon Hee, Nam, Yun Sung, Kang, Da Young, Kim, Hohyun, Lee, Seo Eun, Kim, Hak Rim, Lee, Yong Seong, Jeong, Ji Hoon
Format: Article
Language:English
Subjects:
Administration, Oral
Chromatography
Chromatography, Liquid - methods
Drug Stability
Formates
Human
Humans
Ionization
Liquids
Male
Phenyls
Piperidines - blood
Piperidines - pharmacokinetics
Precursors
Pyridines - blood
Pyridines - pharmacokinetics
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
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Summary:A sensitive and rapid method for quantitation of bepotastine in human plasma has been established using ultra performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC–ESI–MS/MS). Valsartan was used as an internal standard. Bepotastine and internal standard in plasma sample were extracted using ethylacetate (liquid–liquid extraction). A centrifuged upper layer was then evaporated and reconstituted with the mobile phase of acetonitrile—5 mM ammonium formate (pH 3.5) (85:15, v/v). The reconstituted samples were injected into a phenyl column. Using MS/MS in the multiple reaction monitoring mode, bepotastine and valsartan were detected without severe interference from human plasma matrix. Bepotastine produced a protonated precursor ion ([M+H]+) at m/z 389 and a corresponding product ion at m/z 167. And the internal standard produced a protonated precursor ion ([M+H]+) at m/z 436 and a corresponding product ion at m/z 291. Detection of bepotastine in human plasma by the UPLC–ESI–MS/MS method was accurate and precise with a quantitation limit of 0.2 ng/mL. The validation, reproducibility, stability and recovery of the method were evaluated. The method has been successfully applied to pharmacokinetic studies of bepotastine in human plasma.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmt135