Effect of formulation on the rhizosphere competence and biocontrol ability of Trichoderma atroviride C52

• Published in: Plant pathology 2005-04, Vol.54 (2), p.212-218
• Main Authors: McLean, K.L, Swaminathan, J, Frampton, C.M, Hunt, J.S, Ridgway, H.J, Stewart, A
• Format: Article
• Language: English
• Subjects: Allium cepa; Biological and medical sciences; biological control; disease control; Fundamental and applied biological sciences. Psychology; fungal antagonists; fungal diseases of plants; Fungal plant pathogens; mycoparasites; onion white rot; onions; Phytopathology. Animal pests. Plant and forest protection; plant pathogenic fungi; population dynamics; rhizosphere; Sclerotium cepivorum; Trichoderma; Trichoderma atroviride; UP‐PCR band profile
• ISSN: 0032-0862; 1365-3059
• DOI: 10.1111/j.1365-3059.2005.01158.x

The rhizosphere competence of the biological control agent Trichoderma atroviride isolate C52 was studied on onion roots both in the glasshouse and in the field when introduced into soil in a range of formulations. Proliferation of T. atroviride in the rhizosphere was formulation‐dependent. A pellet formulation maintained the fungal concentration at 105 cfu per g soil, whereas solid‐substrate and seed‐coating formulations gave concentrations of 104 and 101 cfu per g soil, respectively. To facilitate rhizosphere‐competence studies, a UP‐PCR band profile generated with primer L45 for isolate C52 was used to enable conclusive identification of T. atroviride C52 when recovered from soil. When isolate C52 was introduced into Sclerotium cepivorum‐infested soil as both pellet and solid‐substrate formulations, there was no statistically significant difference in the disease control between these treatments, but the pellet treatment doubled the percentage of healthy plants compared with the control treatment.