Do the intestinal microbiotas differ between paddlefish (Polyodon spathala) and bighead carp (Aristichthys nobilis) reared in the same pond?

• Published in: Journal of applied microbiology 2014-11, Vol.117 (5), p.1245-1252
• Main Authors: Li, X.M, Zhu, Y.J, Yan, Q.Y, Ringø, E, Yang, D.G
• Format: Article
• Language: English
• Subjects: Actinobacteria; Animals; Aristichthys nobilis; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; bighead carp (Aristichthys nobilis); Biological and medical sciences; Carp; Carps - microbiology; correspondence analysis; filter‐feeding fish; Firmicutes; Fish; Fishes - microbiology; Fundamental and applied biological sciences. Psychology; Fusobacteria; genes; genetic background; histology; intestinal microbiota; intestinal microorganisms; intestines; Intestines - microbiology; microbial communities; Microbiology; Microbiota; paddlefish (Polyodon spathala); physiology; Planctomycetes; Polyodon; Ponds; Proteobacteria; pyrosequencing; rearing; ribosomal RNA; RNA, Ribosomal, 16S - genetics; sequence analysis; Small intestine; species diversity; Verrucomicrobia; Verrucomicrobium
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12626

AIMS: A study was conducted to compare the intestinal microbial compositions of two fish species with similar feeding strategy; paddlefish (Polyodon spathala) and bighead carp (Aristichthys nobilis) reared in the same pond. METHODS AND RESULTS: Age‐0 paddlefish and bighead carp with mean average body lengths of 43·39 ± 2·78 and 19·33 ± 3·68 cm, respectively, were reared with natural prey items in the same pond (20 m²). After 30 days of rearing, the intestinal microbiota of the two fish species was assessed by pyrosequencing of 16S rRNA genes. Interestingly, deviations were observed in the microbial communities of the two fish species according to the alpha‐ and beta‐diversity measurements and detrended correspondence analysis (DCA). Shannon diversity (P = 0·015) and Pielou.evenness (P = 0·035) revealed significant lower diversity of the intestinal microbiota of paddlefish. Moreover, different core intestinal microbiota was noticed in the two fish species. Proteobacteria (57·3%), Firmicutes (11·9%), Fusobacteria (8·9%), Planctomycetes (7·3%), Actinobacteria (6·0%) and Verrucomicrobia (3·2%) were detected in bighead carp, while the dominant phyla in paddlefish intestines were Bacteroidetes (37·0%), Fusobacteria (35·1%), Firmicutes (14·8%) and Proteobacteria (12·6%). CONCLUSIONS: Our results revealed that the intestinal microbiota differed between paddlefish and bighead carp reared in the same pond when fed similar nature food. The potential host factors, such as the genetic background, gut histology and physiology are assumed to be involved in the intestinal bacterial compositions. SIGNIFICANCE AND IMPACT OF THE STUDY: Considering the similar feeding strategy of paddlefish and bighead carp, this study presents basic knowledge for evaluation of the importance of host factors (genetic background and gut anatomy) on intestinal microbial composition.