The development of sequence-tagged sites (STSs) in Lolium perenne L. : the application of primer sets derived from other genera

• Published in: Theoretical and applied genetics 2001-09, Vol.103 (4), p.648-658
• Main Authors: TAYLOR, C, MADSEN, K, BORG, S, MØLLER, M. G, BOELT, B, HOLM, P. B
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Barley; Biological and medical sciences; Classical and quantitative genetics. Population genetics. Molecular genetics; Cloning; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genes; Genetics and breeding of economic plants; Genomes; Grasses; Lolium perenne; Molecular genetics; Phalaris coerulescens; Poaceae; Triticum tauchii
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/PL00002920

Genetic analysis, particularly the development of genetic linkage maps in forage grass species, lags well behind other members of the Poaceae. Comparative mapping within this family has revealed extensive conservation in gene and marker synteny among chromosomes of diverse genera. Recently, the ability to transfer mapped STS markers between barley and wheat has been demonstrated. The transfer of mapped STS markers between cereals and forage grasses could provide PCR-based markers for comparative mapping in these species providing they amplify homologous sequences. In this study, primers derived from three barley genes of defined function and a gene from Phalaris coerulescens were used to amplify homologous fragments in Lolium perenne. Primers derived from two barley and two oat cDNA clones were also tested along with eight barley and two Triticum tauchii STS markers. Twenty one primer pairs derived from 18 loci were tested. Eleven primer pairs (52%) amplified homologous sequences in L. perenne from ten (55%) of the loci targetted. Thirteen new STS markers were generated in L. perenne, of which ten have been mapped in barley or rye and amplify homologous sequences in L. perenne.[PUBLICATION ABSTRACT]