Molecular Cloning and Characterization of a Dermatan-specific N-Acetylgalactosamine 4-O-Sulfotransferase

• Published in: The Journal of biological chemistry 2001-09, Vol.276 (39), p.36344-36353
• Main Authors: Evers, Matthias R., Xia, Guoqing, Kang, Hyung-Gyoo, Schachner, Melitta, Baenziger, Jacques U.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Carbohydrate Sequence; CHO Cells; Chromatography, Gel; Chromosomes, Human, Pair 15; Cloning, Molecular; Cricetinae; Dermatan Sulfate - chemistry; dermatan-4-sulfotransferase-1; DNA, Complementary - metabolism; Dose-Response Relationship, Drug; Exons; Humans; Models, Chemical; Models, Genetic; Molecular Sequence Data; N-acetylgalactosamine-4-O-sulfotransferase; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Protein Binding; Protein Structure, Tertiary; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Sulfotransferases - biosynthesis; Sulfotransferases - chemistry; Sulfotransferases - genetics; Time Factors; Tissue Distribution; Transfection
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M105848200

We have identified and characterized anN-acetylgalactosamine-4-O-sulfotransferase designated dermatan-4-sulfotransferase-1 (D4ST-1) (GenBankTM accession number AF401222) based on its homology to HNK-1 sulfotransferase. The cDNA predicts an open reading frame encoding a type II membrane protein of 376 amino acids with a 43-amino acid cytoplasmic domain and a 316-amino acid luminal domain containing two potential N-linked glycosylation sites. D4ST-1 has significant amino acid identity with HNK-1 sulfotransferase (21.4%),N-acetylgalactosamine-4-O-sulfotransferase 1 (GalNAc-4-ST1) (24.7%),N-acetylgalactosamine-4-O-sulfotransferase 2 (GalNAc-4-ST2) (21.0%), chondroitin-4-O-sulfotransferase 1 (27.3%), and chondroitin-4-O-sulfotransferase 2 (22.8%). D4ST-1 transfers sulfate to the C-4 hydroxyl of β1,4-linked GalNAc that is substituted with an α-linked iduronic acid (IdoUA) at the C-3 hydroxyl. D4ST-1 shows a strong preferencein vitro for sulfate transfer to IdoUAα1,3GalNAcβ1,4 that is flanked by GlcUAβ1,3GalNAcβ1,4 as compared with IdoUAα1,3GalNAcβ1,4 flanked by IdoUAα1,3GalNAcβ1,4. The specificity of D4ST-1 when assayed in vitro suggests that the addition of sulfate to GalNAc occurs immediately after epimerization of GlcUA to IdoUA. The open reading frame of D4ST-1 is encoded by a single exon located on human chromosome 15q14. Northern blot analysis reveals a single 2.4-kilobase transcript. D4ST-1 message is expressed in virtually all tissues at some level but is most highly expressed in pituitary, placenta, uterus, and thyroid. The properties of D4ST-1 indicate that sulfation of the GalNAc moieties in dermatan is mediated by a distinct GalNAc-4-O-sulfotransferase and occurs following epimerization of GlcUA to IdoUA.