The Y Chromosome in the Liverwort Marchantia polymorpha has Accumulated Unique Repeat Sequences Harboring a Male-Specific Gene

The haploid liverwort Marchantia polymorpha has heteromorphic sex chromosomes, an X chromosome in the female and a Y chromosome in the male. We here report on the repetitive structure of the liverwort Y chromosome through the analysis of male-specific P1-derived artificial chromosome (PAC) clones, p...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-07, Vol.98 (16), p.9454-9459
Main Authors: Okada, Sachiko, Sone, Takefumi, Fujisawa, Masaki, Nakayama, Shigeki, Takenaka, Mizuki, Ishizaki, Kimitsune, Kono, Kaoru, Shimizu-Ueda, Yuu, Hanajiri, Tsutomu, Yamato, Katsuyuki T., Fukuzawa, Hideya, Brennicke, Axel, Ohyama, Kanji
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological Sciences
Chromosome Mapping
Chromosome polymorphism
Chromosomes
Chromosomes, Artificial
DNA
DNA, Plant
Evolution
Flowers & plants
Genes
Genomes
Genomics
In Situ Hybridization, Fluorescence
Magnoliopsida - genetics
Marchantia polymorpha
Molecular Sequence Data
Open Reading Frames
Plants
Polymerase Chain Reaction
Proteins
Sequence Homology, Amino Acid
Sex chromosomes
Sexes
Y Chromosome
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Summary:The haploid liverwort Marchantia polymorpha has heteromorphic sex chromosomes, an X chromosome in the female and a Y chromosome in the male. We here report on the repetitive structure of the liverwort Y chromosome through the analysis of male-specific P1-derived artificial chromosome (PAC) clones, pMM4G7 and pMM23-130F12. Several chromosome-specific sequence elements of ≈70 to 400 nt are combined into larger arrangements, which in turn are assembled into extensive Y chromosome-specific stretches. These repeat sequences contribute 2-3 Mb to the Y chromosome based on the observations of three different approaches: fluorescence in situ hybridization, dot blot hybridization, and the frequency of clones containing the repeat sequences in the genomic library. A novel Y chromosome-specific gene family was found embedded among these repeat sequences. This gene family encodes a putative protein with a RING finger motif and is expressed specifically in male sexual organs. To our knowledge, there have been no other reports for an active Y chromosome-specific gene in plants. The chromosome-specific repeat sequences possibly contribute to determining the identity of the Y chromosome in M. polymorpha as well as to maintaining genes required for male functions, as in mammals such as human.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.171304798