Bioaugmentation of Hydrogenispora ethanolica LX-B affects hydrogen production through altering indigenous bacterial community structure

• Published in: Bioresource technology 2016-07, Vol.211, p.319-326
• Main Authors: Yang, Zhiman, Guo, Rongbo, Shi, Xiaoshuang, He, Shuai, Wang, Lin, Dai, Meng, Qiu, Yanling, Dang, Xiaoxiao
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial community shift; Bioaugmentation; Bioreactors - microbiology; Firmicutes - metabolism; Hydrogen - analysis; Hydrogen - metabolism; Hydrogen production; Sewage - microbiology
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.03.097

•The added Hydrogenispora ethanolica LX-B caused a shift in metabolic pathway.•The added H. ethanolica LX-B caused a shift in indigenous bacterial community.•Enhanced hydrogen production was related to change in bacterial community structure. Bioaugmentation can facilitate hydrogen production from complex organic substrates, but it still is unknown how indigenous microbial communities respond to the added bacteria. Here, using a Hydrogenispora ethanolica LX-B (named as LX-B) bioaugmentation experiments, the distribution of metabolites and the responses of indigenous bacterial communities were investigated via batch cultivation (BC) and repeated batch cultivation (RBC). In BC the LX-B/sludge ratio of 0.12 achieved substantial high hydrogen yield, which was over twice that of control. In RBC one-time bioaugmentation and repeated batch bioaugmentation of LX-B resulted in the hydrogen yield that was average 1.2-fold and 0.8-fold higher than that in control, respectively. This improved hydrogen production performance mainly benefited from a shift in composition of the indigenous bacterial community caused by LX-B bioaugmentation. The findings represented an important step in understanding the relationship between bioaugmentation, a shift in bacterial communities, and altered bioreactor performance.