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Mating Type Gene (MAT) and Itraconazole Susceptibility of Trichophyton tonsurans Strains Isolated in Japan
Infection by Trichophyton tonsurans is an emerging fungal epidemic in Japan. Itraconazole (ITZ) and terbinafine have been used for the treatment of this infection for 15 years. However, patients with T. tonsurans infections have been shown to remain uncured or to become reinfected, suggesting that s...
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Published in: | Mycopathologia (1975) 2016-06, Vol.181 (5-6), p.441-444 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Infection by
Trichophyton tonsurans
is an emerging fungal epidemic in Japan. Itraconazole (ITZ) and terbinafine have been used for the treatment of this infection for 15 years. However, patients with
T. tonsurans
infections have been shown to remain uncured or to become reinfected, suggesting that subclinical infection or polyphyletic strains and/or antifungal drug-resistant strains might be occurring in Japan. In this study, PCR analysis was performed to confirm the presence of the mating type locus
MAT
in genomic DNA from 60 Japanese clinical isolates of
T. tonsurans
, and to assess the previously postulated clonal origin of clinical isolates of this species. Antifungal susceptibility testing on isolates also was performed to confirm the absence of strains resistant to ITZ. PCR analysis proved that all 60 strains contained the
MAT1
-
1
allele, while none contained the
MAT1
-
2
allele. As determined by E-test, the mean MIC of ITZ in the 60 strains was 0.023 mg/L (range 0.002–0.125 mg/L). All strains of
T. tonsurans
isolated in Japan were clonal and were not resistant to ITZ. Therefore, dermatophytosis due to
T. tonsurans
is expected to respond to ITZ, since clinical isolates of
T. tonsurans
tested to date have been susceptible to this antifungal. This infection is proliferating as a subclinical infection in Japan. |
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ISSN: | 0301-486X 1573-0832 |
DOI: | 10.1007/s11046-015-9980-4 |