Production of bioactive peptide hydrolysates from deer, sheep, pig and cattle red blood cell fractions using plant and fungal protease preparations

•Hydrolysis of cattle, sheep, deer and pig blood cells using plant and fungal proteases.•In vitro antioxidant activities were determined by ORAC, DPPH and FRAP assays.•Hydrolysates generated with papain had higher antioxidant activities than fungal proteases.•Hydrolysates generated with fungal prote...

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Bibliographic Details
Published in:Food chemistry 2016-07, Vol.202, p.458-466
Main Authors: Bah, Clara S.F., Carne, Alan, McConnell, Michelle A., Mros, Sonya, Bekhit, Alaa El-Din A.
Format: Article
Language:English
Subjects:
Animal blood
Animals
Anti-Bacterial Agents - pharmacology
Antioxidant activity
Antioxidants - pharmacology
Cattle - blood
Deer - blood
Erythrocytes - metabolism
Escherichia coli
Fungal protease
Fungi - enzymology
Hydrolysis
Peptide Hydrolases - metabolism
Plant protease
Protein Hydrolysates - biosynthesis
Protein Hydrolysates - pharmacokinetics
Pseudomonas aeruginosa
Red blood cell fraction
Sheep - blood
Staphylococcus aureus
Swine - blood
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Summary:•Hydrolysis of cattle, sheep, deer and pig blood cells using plant and fungal proteases.•In vitro antioxidant activities were determined by ORAC, DPPH and FRAP assays.•Hydrolysates generated with papain had higher antioxidant activities than fungal proteases.•Hydrolysates generated with fungal proteases exhibited antimicrobial activities. Protease preparations from plant (papain and bromelain) and fungal (FP400 and FPII) sources were used to hydrolyze the red blood cell fractions (RBCFs) separated from deer, sheep, pig, and cattle abattoir-sourced blood. After 1, 2, 4 and 24h of hydrolysis, the antioxidant and antibacterial activities of the peptide hydrolysates obtained were investigated. The increase in trichloroacetic acid-soluble peptides over the hydrolysis period was examined using the o-phthaldialdehyde (OPA) assay and the hydrolysis profiles were illustrated using SDS–PAGE. Papain generated RBCF hydrolysates exhibited higher ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) compared to those generated with bromelain, FP400 and FPII. At certain concentrations, 24h hydrolysates of RBCF using FP400 and FPII were able to inhibit the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results indicated that the use of proteases from plant or fungal sources can produce animal blood hydrolysates with antioxidant and antimicrobial activities.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2016.02.020