Genotypic and Phenotypic Identification of Aeromonas Species and Cpha-Mediated Carbapenem Resistance in Queensland, Australia

Abstract Infection caused by Aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. Carbapenem resistance due to metallo-beta-lactamase, CphA encoded by the cphA gene is a significant problem. This study defines Aeromonas spp. causing clinical disease in Queensland, A...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2016-05, Vol.85 (1), p.98-101
Main Authors: Sinclair, Holly A, Heney, Claire, Sidjabat, Hanna E, George, Narelle M, Bergh, Haakon, Anuj, Snehal N, Nimmo, Graeme R, Paterson, D.L
Format: Article
Language:English
Subjects:
Aeromonas
Aeromonas - classification
Aeromonas - drug effects
Aeromonas - genetics
Aeromonas - isolation & purification
Aeromonas bestiarum
Aeromonas caviae
Aeromonas hydrophila
Aeromonas jandaei
Aeromonas veronii
Anti-Bacterial Agents - pharmacology
Australia
Bacterial Typing Techniques
beta-Lactam Resistance
beta-Lactamases - genetics
Carbapenem resistance
Carbapenems - pharmacology
CphA
Genotype
Gram-Negative Bacterial Infections - diagnosis
Gram-Negative Bacterial Infections - microbiology
Humans
Infectious Disease
Internal Medicine
Microbial Sensitivity Tests
Sensitivity and Specificity
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Summary:Abstract Infection caused by Aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. Carbapenem resistance due to metallo-beta-lactamase, CphA encoded by the cphA gene is a significant problem. This study defines Aeromonas spp. causing clinical disease in Queensland, Australia. Phenotypic tests for carbapenemase detection were assessed. One hundred Aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2) and sputum (1) were characterized by rpoB and gyrB sequencing. Meropenem susceptibility by VITEK2, disk diffusion and E-test MIC were determined. Carbapenemase production was assessed by Carba NP test and cphA by PCR. Gene sequencing identified isolates as A. dhakensis (39), A. veronii (21), A. hydrophila (20), A. caviae (14), A. jandaei (4), A. bestiarum (1), and A. sanarellii (1). Disk diffusion and E-test failed to detect resistance in isolates with presence of cphA . Carba NP performed with 97.4% sensitivity and 95.7% specificity. Carbapenem resistance gene cphA was detected in A. veronii (21; 100%), A. hydrophila (18; 90%), A. dhakensis (34; 87.2%), A. jandaei (3; 75%), and A. bestiarum (1; 100%) but not A. caviae . We found A. dhakensis was the predominant species, a previously unrecognized pathogen in this region.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2016.02.005