Metagenome changes in the biogas producing community during anaerobic digestion of rice straw

•Metagenome was sequenced to profile the microbial community and metabolism.•Acid producing bacteria were abundant in sour digester.•Abundance of methanogens was more prominent in healthy stage. The present investigation was undertaken to study the microbial community succession in a sour and health...

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Bibliographic Details
Published in:Bioresource technology 2016-08, Vol.213, p.50-53
Main Authors: Pore, Soham D., Shetty, Deepa, Arora, Preeti, Maheshwari, Sneha, Dhakephalkar, Prashant K.
Format: Article
Language:English
Subjects:
Acidogenesis
Anaerobiosis
Bacteria - genetics
Biofuels - microbiology
Euryarchaeota - genetics
Fatty Acids, Volatile - metabolism
Healthy digester
High-Throughput Nucleotide Sequencing
Metagenome
Metagenomics
Methane - metabolism
Methanogenesis
Microbial Consortia
Oryza - microbiology
Oxidoreductases - genetics
Sour digester
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Summary:•Metagenome was sequenced to profile the microbial community and metabolism.•Acid producing bacteria were abundant in sour digester.•Abundance of methanogens was more prominent in healthy stage. The present investigation was undertaken to study the microbial community succession in a sour and healthy digester. Ion torrent next-generation sequencing (NGS)-based metagenomic approach indicated abundance of hydrolytic bacteria and exclusion of methanogens and syntrophic bacteria in sour digester. Functional gene analysis revealed higher abundance of enzymes involved in acidogenesis and lower abundance of enzymes associated with methanogenesis like Methyl coenzyme M-reductase, F420 dependent reductase and Formylmethanofuran dehydrogenase in sour digester. Increased abundance of methanogens (Methanomicrobia) and genes involved in methanogenesis was observed in the restored/healthy digester highlighting revival of pH sensitive methanogenic community.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.03.045