Nucleoplasmic Lamin A/C and Polycomb group of proteins: An evolutionarily conserved interplay

Nuclear lamins are the main components of the nuclear lamina at the nuclear periphery, providing mechanical support to the nucleus. However, recent findings suggest that lamins also reside in the nuclear interior, as a distinct and dynamic pool with critical roles in transcriptional regulation. In o...

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Bibliographic Details
Published in:Nucleus (Austin, Tex.) Tex.), 2016-04, Vol.7 (2), p.103-111
Main Authors: Marullo, F., Cesarini, E., Antonelli, L., Gregoretti, F., Oliva, G., Lanzuolo, C.
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Cell Differentiation
Cell Nucleus - metabolism
Evolution, Molecular
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HEK293 Cells
Humans
Lamin A/C
Lamin Type A - metabolism
Myoblasts - cytology
nuclear architecture
nucleoplasm
Polycomb
Polycomb-Group Proteins - metabolism
Protein Binding
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Summary:Nuclear lamins are the main components of the nuclear lamina at the nuclear periphery, providing mechanical support to the nucleus. However, recent findings suggest that lamins also reside in the nuclear interior, as a distinct and dynamic pool with critical roles in transcriptional regulation. In our work we found a functional and evolutionary conserved crosstalk between Lamin A/C and the Polycomb group (PcG) of proteins, this being required for the maintenance of the PcG repressive functions. Indeed, Lamin A/C knock-down causes PcG foci dispersion and defects in PcG-mediated higher order structures, thereby leading to impaired PcG mediated transcriptional repression. By using ad-hoc algorithms for image analysis and PLA approaches we hereby show that PcG proteins are preferentially located in the nuclear interior where they interact with nucleoplasmic Lamin A/C. Taken together, our findings suggest that nuclear components, such as Lamin A/C, functionally interact with epigenetic factors to ensure the correct transcriptional program maintenance.
ISSN:1949-1034
1949-1042
DOI:10.1080/19491034.2016.1157675