Local cerebral hypothermia induced by selective infusion of cold lactated ringer's: a feasibility study in rhesus monkeys

• Published in: Neurological research (New York) 2016-06, Vol.38 (6), p.545-552
• Main Authors: Wang, Bincheng, Wu, Di, Dornbos III, David, Shi, Jingfei, Ma, Yanhui, Zhang, Mo, Liu, Yumei, Chen, Jian, Ding, Yuchuan, Luo, Yinghao, Ji, Xunming
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Angiography, Digital Subtraction; Animals; Blood Pressure - physiology; Body Temperature - physiology; Brain - diagnostic imaging; Brain - physiology; Cerebrovascular Circulation - physiology; Cold Temperature; Digital subtraction angiography (DSA); Disease Models, Animal; Feasibility Studies; Hypothermia, Induced - methods; Image Processing, Computer-Assisted; Isotonic Solutions - administration & dosage; Macaca mulatta; Magnetic Resonance Imaging; Male; Middle cerebral artery (MCA); Middle Cerebral Artery - physiology; Neurologic Examination; Selective hypothermia; Time Factors; Transcranial Doppler (TCD); Ultrasonography, Doppler, Transcranial
• ISSN: 0161-6412; 1743-1328
• DOI: 10.1080/01616412.2016.1187827

Background and Objective: Hypothermia has shown promise as a neuroprotective strategy for stroke. The use of whole body hypothermia has limited clinical utility due to many severe side effects. Selective brain cooling, or local brain hypothermia, has been previously proposed as an alternative treatment strategy. This study investigated the safety, feasibility, and efficacy of selective brain hypothermia induced by local infusion of ice-cold lactated Ringer's solution in rhesus monkeys. Methods: Eight male rhesus monkeys were used in this study. Brain temperature in the territory supplied by middle cerebral artery (MCA) was reduced by infusing 100 mL of ice-cold (0 °C) lactated Ringer's solution over 20 min via a micro-catheter placed in the proximal MCA (n = 4). Vital signs and the temperature of the brain and rectum were monitored before and after infusion. Transcranial Doppler, Magnetic resonance imaging (MRI), and digital subtraction angiography (DSA) were used to evaluate cerebral blood flow, cerebrovascular reactivity (CVR), cerebral edema, and vasospasm. Another cohort of rhesus monkeys (n = 4) were used as systemic cooling controls. Results: Oxygen saturation, blood pressure, heart rate, and hematologic analysis of the two groups remained within the normal range after infusion. Mild cerebral hypothermia (