Lymphoblast-derived integration-free iPS cell line from a 69-year-old male

Human lymphoblast cells were used to generate integration-free induced pluripotent stem (iPS) cells employing episomal plasmids expressing OCT4, SOX2, NANOG, LIN28, C-MYC and L-MYC. The derived iPS cells were defined as pluripotent based on (i) expression of pluripotent-associated markers, (ii) embr...

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Bibliographic Details
Published in:Stem cell research 2016-01, Vol.16 (1), p.29-31
Main Authors: Schröter, Friederike, Sleegers, Kristel, Bohndorf, Martina, Wruck, Wasco, Van Broeckhoven, Christine, Adjaye, James
Format: Article
Language:English
Subjects:
Aged
Cell Culture Techniques - methods
Cell Line
Humans
Induced Pluripotent Stem Cells - cytology
Karyotyping
Lymphocytes - cytology
Male
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:Human lymphoblast cells were used to generate integration-free induced pluripotent stem (iPS) cells employing episomal plasmids expressing OCT4, SOX2, NANOG, LIN28, C-MYC and L-MYC. The derived iPS cells were defined as pluripotent based on (i) expression of pluripotent-associated markers, (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptomes of the iPS cell line and the human embryonic stem cell line H1 with a Pearson correlation of 0.95.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2015.11.016