Assessment of different functionalization methods for grafting a protein to an alumina-covered biosensor

The specificity of biosensors is typically obtained by surface biofunctionalization, which enables the selective binding of biomolecules. This critical step is sensitive to the nature of materials and to the overall experimental conditions. Here, we provide a comprehensive study of several biofuncti...

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Bibliographic Details
Published in:Biofabrication 2014-09, Vol.6 (3), p.035007-035007
Main Authors: Van Overstraeten-Schlögel, N, Lefèvre, O, Couniot, N, Flandre, D
Format: Article
Language:English
Subjects:
alumina
Aluminum oxide
Aluminum Oxide - chemistry
Assessments
Biochemistry - instrumentation
Biochemistry - methods
biofunctionalization
Biomolecules
Biosensing Techniques - instrumentation
Biosensors
Covering
Grafting
Maleimides - chemistry
methods
protein
Proteins
Proteins - chemistry
Respiratory Syncytial Viruses - chemistry
Respiratory Syncytial Viruses - isolation & purification
Selective binding
sensor
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Summary:The specificity of biosensors is typically obtained by surface biofunctionalization, which enables the selective binding of biomolecules. This critical step is sensitive to the nature of materials and to the overall experimental conditions. Here, we provide a comprehensive study of several biofunctionalization methods, including the layer-by-layer technique and both the gas-phase and liquid-phase silanizations, and we propose a new maleimide-based protocol for grafting a protein to a sensor covered by alumina. This method was then validated by making a respiratory syncitial virus-specific biosensor.
ISSN:1758-5082
1758-5090
DOI:10.1088/1758-5082/6/3/035007